Product nameAnti-RRAGC antibody
See all RRAGC primary antibodies
DescriptionRabbit polyclonal to RRAGC
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human RRAGC aa 1-250.
MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGG GCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIY KDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDY MEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRAN DDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLNIF
Database link: Q9HB90
- WB: HeLa, MCF7 and A549 whole cell lysates. IHC-P: Human adrenal gland and small intestine tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionHas guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
Cellular localizationCytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
- Information by UniProt
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
All lanes : Anti-RRAGC antibody (ab230184) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Paraffin-embedded human adrenal gland tissue stained for RRAGC with ab230184 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for RRAGC with ab230184 at 1/100 dilution in immunohistochemical analysis.
ab230184 has not yet been referenced specifically in any publications.