Key features and details
- Rabbit polyclonal to RRAGC - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Hamster, Human
- Isotype: IgG
Product nameAnti-RRAGC antibody - BSA and Azide free
See all RRAGC primary antibodies
DescriptionRabbit polyclonal to RRAGC - BSA and Azide free
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Hamster, Human
Full length native protein (purified) (GST-tag) corresponding to Human RRAGC aa 1-399.
MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGG GCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIY KDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDY MEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRAN DDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIF ISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYG LKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKG LIDYNFHCFRKAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI
Database link: Q9HB90
- HeLa, MCF7, MOLT4, NIH 3T3 and CHO cell lysates; HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
ab206864 is filter-sterilized
Concentration information loading...
PurityProtein A purified
Purification notesab206864 is a purified IgG fraction.
Our Abpromise guarantee covers the use of ab206864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 44 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionHas guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
Cellular localizationCytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
- Information by UniProt
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
All lanes : Anti-RRAGC antibody - BSA and Azide free (ab206864) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : CHO cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : anti-rabbit IgG conjugated with HRP at 1/10000 dilution
Predicted band size: 44 kDa
Immunofluorecent analysis of HeLa cells (fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100) labeling RRAGC with ab206864 at a 1/100 dilution. Secondary staining with anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (red), was used at 1/1000 dilution. DNA was stained with DAPI (blue).
ab206864 has not yet been referenced specifically in any publications.