Key features and details
- Rabbit polyclonal to RRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RRAS antibody
See all RRAS primary antibodies
DescriptionRabbit polyclonal to RRAS
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human RRAS aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human RRAS protein (ab114612) can be used as a positive control in WB. This antibody gave a positive signal in human skeletal muscle as well as the following whole cell lysates: HeLa; HT29; DU145; PC3; A549.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulates the organization of the actin cytoskeleton.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
Cellular localizationCell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine.
- Information by UniProt
- Oncogene RRAS antibody
- p23 antibody
- R Ras antibody
All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HT29 (Human colon adenocarcinoma) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 5 : PC3 whole cell lysate (ab3954)
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab82356 has not yet been referenced specifically in any publications.