Overview

  • Product name

  • Description

    Rabbit polyclonal to RRAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RRAS aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93071)

  • Positive control

    • Recombinant Human RRAS protein (ab114612) can be used as a positive control in WB. This antibody gave a positive signal in human skeletal muscle as well as the following whole cell lysates: HeLa; HT29; DU145; PC3; A549.

Properties

Applications

Our Abpromise guarantee covers the use of ab82356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/250. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Regulates the organization of the actin cytoskeleton.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Ras family.
    • Cellular localization

      Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine.
    • Information by UniProt
    • Database links

    • Alternative names

      • Oncogene RRAS antibody
      • p23 antibody
      • R Ras antibody
      • Ras related Protein antibody
      • Ras related protein R Ras antibody
      • Ras-related protein R-Ras antibody
      • related RAS viral (r ras) oncogene homolog antibody
      • RRAS antibody
      • RRAS_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilution

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HT29 (Human colon adenocarcinoma) Whole Cell Lysate
      Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
      Lane 5 : PC3 whole cell lysate (ab3954)
      Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 23 kDa
      Observed band size: 24 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    References

    ab82356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82356.
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