Overview

  • Product name

  • Description

    Rabbit polyclonal to RRM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human RRM1 aa 742-792. The exact sequence is proprietary.
    Database link: P23921

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.

Target

  • Function

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • Pathway

    Genetic information processing; DNA replication.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Images

  • All lanes : Anti-RRM1 antibody (ab226391) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds
  • RRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer;v1.0 mg for IP, 20% of IP loaded) with ab226389 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226391 at 0.4 µg/ml.

    Lane 1: ab226391 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab226391 has not yet been referenced specifically in any publications.

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