Key features and details
- Rabbit polyclonal to RRM1
- Suitable for: IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RRM1 antibody
See all RRM1 primary antibodies
DescriptionRabbit polyclonal to RRM1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Synthetic peptide within Human RRM1 aa 742-792. The exact sequence is proprietary.
Database link: P23921
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionProvides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
PathwayGenetic information processing; DNA replication.
Sequence similaritiesBelongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain.
- Information by UniProt
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
All lanes : Anti-RRM1 antibody (ab226391) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
RRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer: 1.0 mg for IP, 20% of IP loaded) with ab226389 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226391 at 0.4 µg/ml.
Lane 1: ab226391 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226391 has not yet been referenced specifically in any publications.