Product nameAnti-RRM1 antibody
See all RRM1 primary antibodies
DescriptionRabbit polyclonal to RRM1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Synthetic peptide within Human RRM1 aa 742-792. The exact sequence is proprietary.
Database link: P23921
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 90 kDa.|
FunctionProvides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
PathwayGenetic information processing; DNA replication.
Sequence similaritiesBelongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain.
- Information by UniProt
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
All lanes : Anti-RRM1 antibody (ab226391) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
RRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer;v1.0 mg for IP, 20% of IP loaded) with ab226389 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226391 at 0.4 µg/ml.
Lane 1: ab226391 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226391 has not yet been referenced specifically in any publications.