
Recombinant Anti-RRM1 antibody [EPR8482] (ab133690)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RRM1 antibody [EPR8482]
See all RRM1 primary antibodies -
Description
Rabbit monoclonal [EPR8482] to RRM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RRM1 aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, HT29, RAW 264.7 and NIH 3T3 cell lysates; Human placenta and kidney tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.24 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8482 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. -
Pathway
Genetic information processing; DNA replication. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6240 Human
- Entrez Gene: 20133 Mouse
- Entrez Gene: 685579 Rat
- Omim: 180410 Human
- SwissProt: P23921 Human
- SwissProt: P07742 Mouse
- Unigene: 445705 Human
- Unigene: 197486 Mouse
see all -
Alternative names
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling RRM1 with ab133690 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RRM1 with ab133690 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Datasheets and documents
References
ab133690 has not yet been referenced specifically in any publications.