RecombinantRabMAb

Recombinant Anti-RRM1 antibody [EPR8482] (ab133690)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8482] to RRM1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RRM1 antibody [EPR8482]
    See all RRM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8482] to RRM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RRM1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, HT29, RAW 264.7 and NIH 3T3 cell lysates; Human placenta and kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    • Pathway

      Genetic information processing; DNA replication.

    • Sequence similarities

      Belongs to the ribonucleoside diphosphate reductase large chain family.
      Contains 1 ATP-cone domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • R1 antibody
      • Ribonucleoside diphosphate reductase large subunit antibody
      • Ribonucleoside diphosphate reductase M1 chain antibody
      • Ribonucleoside diphosphate reductase subunit M1 antibody
      • Ribonucleoside reductase, large subunit antibody
      • Ribonucleoside-diphosphate reductase large subunit antibody
      • Ribonucleoside-diphosphate reductase subunit M1 antibody
      • Ribonucleotide reductase large chain antibody
      • Ribonucleotide reductase large subunit antibody
      • Ribonucleotide reductase M1 antibody
      • Ribonucleotide reductase M1 polypeptide antibody
      • Ribonucleotide reductase R1 subunit antibody
      • Ribonucleotide reductase, M1 subunit antibody
      • RIR 1 antibody
      • RIR1 antibody
      • RIR1_HUMAN antibody
      • RR 1 antibody
      • RR1 antibody
      • RRM 1 antibody
      • RRM1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling RRM1 with ab133690 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RRM1 with ab133690 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RRM1 antibody [EPR8482] (ab133690)
      Western blot - Anti-RRM1 antibody [EPR8482] (ab133690)
      All lanes : Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : RAW 264.7 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 90 kDa

    • OI-RD Scanning - Anti-RRM1 antibody [EPR8482] (ab133690)
      OI-RD Scanning - Anti-RRM1 antibody [EPR8482] (ab133690)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-RRM1 antibody [EPR8482] (ab133690)
      Anti-RRM1 antibody [EPR8482] (ab133690)

    References (0)

    Publishing research using ab133690? Please let us know so that we can cite the reference in this datasheet.

    ab133690 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Antibody worked well then stopped working after being stored at -20C.

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    Answer

    Thank you for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial of ab81085 on the order ***, which should arrive on Tuesday.

    Please keep me updated about the results with this antibody, and if there is anything else that we can do for you please don't hesitate to ask.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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