Recombinant
RabMAb

Recombinant Anti-RRM1 antibody [EPR8482] (ab133690)

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Overview

  • Product name

    Anti-RRM1 antibody [EPR8482]
    See all RRM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8482] to RRM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RRM1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, HT29, RAW 264.7 and NIH 3T3 cell lysates; Human placenta and kidney tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    • Pathway

      Genetic information processing; DNA replication.

    • Sequence similarities

      Belongs to the ribonucleoside diphosphate reductase large chain family.
      Contains 1 ATP-cone domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • R1 antibody
      • Ribonucleoside diphosphate reductase large subunit antibody
      • Ribonucleoside diphosphate reductase M1 chain antibody
      • Ribonucleoside diphosphate reductase subunit M1 antibody
      • Ribonucleoside reductase, large subunit antibody
      • Ribonucleoside-diphosphate reductase large subunit antibody
      • Ribonucleoside-diphosphate reductase subunit M1 antibody
      • Ribonucleotide reductase large chain antibody
      • Ribonucleotide reductase large subunit antibody
      • Ribonucleotide reductase M1 antibody
      • Ribonucleotide reductase M1 polypeptide antibody
      • Ribonucleotide reductase R1 subunit antibody
      • Ribonucleotide reductase, M1 subunit antibody
      • RIR 1 antibody
      • RIR1 antibody
      • RIR1_HUMAN antibody
      • RR 1 antibody
      • RR1 antibody
      • RRM 1 antibody
      • RRM1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling RRM1 with ab133690 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RRM1 with ab133690 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RRM1 antibody [EPR8482] (ab133690)
      Western blot - Anti-RRM1 antibody [EPR8482] (ab133690)
      All lanes : Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : RAW 264.7 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 90 kDa

    • Other - Anti-RRM1 antibody [EPR8482] (ab133690)
      Other - Anti-RRM1 antibody [EPR8482] (ab133690)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133690 has not yet been referenced specifically in any publications.

    Publishing research using ab133690? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Antibody worked well then stopped working after being stored at -20C.

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    Answer

    Thank you for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial of ab81085 on the order ***, which should arrive on Tuesday.

    Please keep me updated about the results with this antibody, and if there is anything else that we can do for you please don't hesitate to ask.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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