Recombinant Anti-RRM1 antibody [EPR8483] (ab137114)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-6-anti-rrm1-antibody-epr8483-immunohistochemistry-lung-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8483] to RRM1
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RRM1 antibody [EPR8483]
See all RRM1 primary antibodies -
Description
Rabbit monoclonal [EPR8483] to RRM1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human RRM1 aa 700-800. The exact sequence is proprietary.
Database link: P23921 -
Positive control
- WB: HT-29, A549, MCF7 and HeLa whole cell lysate (ab150035), Mouse colon, Rat brain, and Rat colon lysates. IHC-P: human lung and colon carcinoma tissues. ICC/IF: HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8483 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 90 kDa.
|
|
| Flow Cyt |
1/100.
|
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 90 kDa. |
|
Flow Cyt
1/100. |
|
ICC/IF
1/100 - 1/250. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Target
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Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. -
Pathway
Genetic information processing; DNA replication. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6240 Human
- Entrez Gene: 20133 Mouse
- Entrez Gene: 685579 Rat
- Omim: 180410 Human
- SwissProt: P23921 Human
- SwissProt: P07742 Mouse
- Unigene: 445705 Human
- Unigene: 197486 Mouse
see all -
Alternative names
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling RRM1 with purified ab137114 at 1/1000 dilution (0.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-365432-anti-rrm1-antibody-epr8483-westernblot-human-mouse-rat.jpg)
Western blot - Anti-RRM1 antibody [EPR8483] (ab137114)All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution (Purified)
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse colon lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat colon lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-7-anti-rrm1-antibody-epr8483-immunocytochemistry-ht29-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [EPR8483] (ab137114)Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling RRM1 with purified ab137114 at 1/100 dilution (9.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-151729-anti-rrm1-antibody-epr8483-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-271140-anti-rrm1-antibody-epr8483-flow-cytometry.jpg)
Flow Cytometry - Anti-RRM1 antibody [EPR8483] (ab137114)unpurified ab137114 staining RRM1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Western blot - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-151731-anti-rrm1-antibody-epr8483-western-blot.jpg)
Western blot - Anti-RRM1 antibody [EPR8483] (ab137114)All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution ((unpurified))
Lane 1 : HT29 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-151728-anti-rrm1-antibody-epr8483-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8483] (ab137114)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RRM1 with unpurified ab137114 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-151730-anti-rrm1-antibody-epr8483-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [EPR8483] (ab137114)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, CanadaUnpurified ab137114 (1/200) staining RRM1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
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![Anti-RRM1 antibody [EPR8483] (ab137114)](https://www.abcam.com/ps/products/137/ab137114/Images/ab137114-9-benefits-of-recombinant-antibodies.png)
Anti-RRM1 antibody [EPR8483] (ab137114)
Protocols
References (8)
ab137114 has been referenced in 8 publications.
- Zhou J et al. Identification of chemoresistance-related mRNAs based on gemcitabine-resistant pancreatic cancer cell lines. Cancer Med 9:1115-1130 (2020). PubMed: 31823522
- Okonska A et al. Functional Genomic Screen in Mesothelioma Reveals that Loss of Function of BRCA1-Associated Protein 1 Induces Chemoresistance to Ribonucleotide Reductase Inhibition. Mol Cancer Ther 19:552-563 (2020). PubMed: 31619462
- Qi W et al. Inhibition of Wee1 sensitizes AML cells to ATR inhibitor VE-822-induced DNA damage and apoptosis. Biochem Pharmacol 164:273-282 (2019). PubMed: 31014753
- Shinoda S et al. Deferasirox, an oral iron chelator, with gemcitabine synergistically inhibits pancreatic cancer cell growth in vitro and in vivo. Oncotarget 9:28434-28444 (2018). PubMed: 29983871
- Kawamoto M et al. Combined Gemcitabine and Metronidazole Is a Promising Therapeutic Strategy for Cancer Stem-like Cholangiocarcinoma. Anticancer Res 38:2739-2748 (2018). PubMed: 29715094
- Liang M et al. CHK1 inhibition sensitizes pancreatic cancer cells to gemcitabine via promoting CDK-dependent DNA damage and ribonucleotide reductase downregulation. Oncol Rep 39:1322-1330 (2018). PubMed: 29286153
- Liu S et al. Inhibition of ATR potentiates the cytotoxic effect of gemcitabine on pancreatic cancer cells through enhancement of DNA damage and abrogation of ribonucleotide reductase induction by gemcitabine. Oncol Rep 37:3377-3386 (2017). PubMed: 28440428
- Kuny CV & Kalejta RF Human Cytomegalovirus Can Procure Deoxyribonucleotides for Viral DNA Replication in the Absence of Retinoblastoma Protein Phosphorylation. J Virol 90:8634-43 (2016). PubMed: 27440891
