Product nameAnti-RRM1 antibody [EPR8483]
See all RRM1 primary antibodies
DescriptionRabbit monoclonal [EPR8483] to RRM1
Tested applicationsSuitable for: WB, Flow Cyt, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RRM1 aa 700-800. The exact sequence is proprietary.
Database link: P23921
- A549, HT29, MCF7 and HeLa whole cell lysate (ab150035); Human colon carcinoma and lung carcinoma tissues.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab137114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 90 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||1/10 - 1/100.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProvides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
PathwayGenetic information processing; DNA replication.
Sequence similaritiesBelongs to the ribonucleoside diphosphate reductase large chain family.
Contains 1 ATP-cone domain.
- Information by UniProt
- R1 antibody
- Ribonucleoside diphosphate reductase large subunit antibody
- Ribonucleoside diphosphate reductase M1 chain antibody
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.
ab137114 staining RRM1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution
Lane 1 : HT29 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
ab137114 (1/200) staining RRM1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.
This product has been referenced in:
- Shinoda S et al. Deferasirox, an oral iron chelator, with gemcitabine synergistically inhibits pancreatic cancer cell growth in vitro and in vivo. Oncotarget 9:28434-28444 (2018). Read more (PubMed: 29983871) »
- Kawamoto M et al. Combined Gemcitabine and Metronidazole Is a Promising Therapeutic Strategy for Cancer Stem-like Cholangiocarcinoma. Anticancer Res 38:2739-2748 (2018). Read more (PubMed: 29715094) »