Overview

  • Product name

    Anti-RRM1 antibody [EPR8483]
    See all RRM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8483] to RRM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RRM1 aa 700-800. The exact sequence is proprietary.
    Database link: P23921

  • Positive control

    • A549, HT29, MCF7 and HeLa whole cell lysate (ab150035); Human colon carcinoma and lung carcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 90 kDa.
Flow Cyt Use at an assay dependent concentration.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • Pathway

    Genetic information processing; DNA replication.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.

  • ab137114 staining RRM1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • All lanes : Anti-RRM1 antibody [EPR8483] (ab137114) at 1/10000 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 90 kDa

  • ab137114 (1/200) staining RRM1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RRM1 with ab137114 at 1/100 dilution.

References

This product has been referenced in:

  • Shinoda S  et al. Deferasirox, an oral iron chelator, with gemcitabine synergistically inhibits pancreatic cancer cell growth in vitro and in vivo. Oncotarget 9:28434-28444 (2018). Read more (PubMed: 29983871) »
  • Kawamoto M  et al. Combined Gemcitabine and Metronidazole Is a Promising Therapeutic Strategy for Cancer Stem-like Cholangiocarcinoma. Anticancer Res 38:2739-2748 (2018). Read more (PubMed: 29715094) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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