Overview

  • Product name

    Anti-RRM1 antibody [SP167]
    See all RRM1 primary antibodies
  • Description

    Rabbit monoclonal [SP167] to RRM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human RRM1 aa 300-400 (internal sequence). The exact sequence is proprietary.
    Database link: P23921

  • Positive control

    • Human lung adenocarcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab135383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • Pathway

    Genetic information processing; DNA replication.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling RRM1 with ab135383 antibody at a dilution of 1/100.

  • Flow cytometric analysis of rabbit anti-RRM1 (SP167) antibody ab135383 (1/400) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

  • ICC/IF image of ab135383 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135383 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Sasaki N  et al. Stemness and anti-cancer drug resistance in ABCG2 highly expressed pancreatic cancer is induced on 3D-culture condition. Cancer Sci N/A:N/A (2018). Read more (PubMed: 29444383) »
See 1 Publication for this product

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