Recombinant
RabMAb

Recombinant Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

Overview

  • Product name

    Anti-RRM1 antibody [SP167] - BSA and Azide free
    See all RRM1 primary antibodies
  • Description

    Rabbit monoclonal [SP167] to RRM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human RRM1 aa 300-400 (internal sequence). The exact sequence is proprietary.
    Database link: P23921

  • General notes

    Ab240093 is the carrier-free version of ab135383. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240093 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • Pathway

    Genetic information processing; DNA replication.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human lung adenocarcinoma tissue labeling RRM1 with ab135383 antibody at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135383).

  • Flow cytometric analysis of rabbit anti-RRM1 (SP167) antibody ab135383 (1/400) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135383).

  • ICC/IF image of ab135383 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135383 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135383).

References

ab240093 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240093.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up