Overview

  • Product name
    Anti-RRM2 antibody
    See all RRM2 primary antibodies
  • Description
    Rabbit polyclonal to RRM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-259 of RRM2 (P31350).

  • Positive control
    • 293T, A431, H1299, HeLa, Molt4 whole cell lysates; A549 cells; Human Cal27 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
  • Pathway
    Genetic information processing; DNA replication.
  • Sequence similarities
    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Post-translational
    modifications
    Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • cb111 antibody
      • chunp6884 antibody
      • etID309896.20 antibody
      • R2 antibody
      • reductase M2 polypeptide variant 1 antibody
      • reductase M2 polypeptide variant 2 antibody
      • reductase M2 polypeptide variant 3a antibody
      • reductase M2 polypeptide variant 3b antibody
      • reductase M2 polypeptide variant 3c antibody
      • reductase M2 polypeptide variant 3d antibody
      • Ribonucleoside-diphosphate reductase subunit M2 antibody
      • Ribonucleotide reductase M2 antibody
      • Ribonucleotide reductase M2 polypeptide antibody
      • Ribonucleotide reductase M2 subunit antibody
      • ribonucleotide reductase protein r2 class I antibody
      • Ribonucleotide reductase regulatory subunit M2 antibody
      • Ribonucleotide reductase small chain antibody
      • Ribonucleotide reductase small subunit antibody
      • Ribonucleotide reductase, R2 subunit antibody
      • RIR2_HUMAN antibody
      • RR2 antibody
      • RR2M antibody
      • RRM2 antibody
      see all

    Images

    • Western blot - Anti-RRM2 antibody (ab154964)
      Western blot - Anti-RRM2 antibody (ab154964)
      Anti-RRM2 antibody (ab154964) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 45 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)
      Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling RRM2 with ab154964 at 1/500.
    • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)
      Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)
      Immunofluorescence analysis of methanol fixed A549 cells labeling RRM2 with ab154964 at 1/500. Lower panel shows costaining with Hoechst 33342.

    References

    ab154964 has not yet been referenced specifically in any publications.

    Publishing research using ab154964? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab154964.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up