Anti-RRM2 antibody (ab154964)
Key features and details
- Rabbit polyclonal to RRM2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RRM2 antibody
See all RRM2 primary antibodies -
Description
Rabbit polyclonal to RRM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-259 of RRM2 (P31350).
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Positive control
- 293T, A431, H1299, HeLa, Molt4 whole cell lysates; A549 cells; Human Cal27 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Cow
Zebrafish
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. -
Pathway
Genetic information processing; DNA replication. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase small chain family. -
Post-translational
modificationsPhosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6241 Human
- Entrez Gene: 100330864 Zebrafish
- Entrez Gene: 30733 Zebrafish
- Omim: 180390 Human
- SwissProt: P31350 Human
- SwissProt: P79733 Zebrafish
- Unigene: 226390 Human
- Unigene: 75098 Zebrafish
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Alternative names
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
see all
Images
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Western blot - Anti-RRM2 antibody (ab154964)Anti-RRM2 antibody (ab154964) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling RRM2 with ab154964 at 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)Immunofluorescence analysis of methanol fixed A549 cells labeling RRM2 with ab154964 at 1/500. Lower panel shows costaining with Hoechst 33342.
Protocols
References (1)
ab154964 has been referenced in 1 publication.
- Hu CM et al. Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation. FASEB J 33:2017-2025 (2019). PubMed: 30199284
