Anti-RRM2 antibody (ab154964)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RRM2 antibody
See all RRM2 primary antibodies -
Description
Rabbit polyclonal to RRM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to a region within amino acids 1-259 of RRM2 (P31350).
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Positive control
- 293T, A431, H1299, HeLa, Molt4 whole cell lysates; A549 cells; Human Cal27 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. -
Pathway
Genetic information processing; DNA replication. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase small chain family. -
Post-translational
modificationsPhosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6241 Human
- Entrez Gene: 100330864 Zebrafish
- Entrez Gene: 30733 Zebrafish
- Omim: 180390 Human
- SwissProt: P31350 Human
- SwissProt: P79733 Zebrafish
- Unigene: 226390 Human
- Unigene: 75098 Zebrafish
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Alternative names
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
see all
Images
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Western blot - Anti-RRM2 antibody (ab154964)Anti-RRM2 antibody (ab154964) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling RRM2 with ab154964 at 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)Immunofluorescence analysis of methanol fixed A549 cells labeling RRM2 with ab154964 at 1/500. Lower panel shows costaining with Hoechst 33342.
Protocols
References
This product has been referenced in:
- Hu CM et al. Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation. FASEB J 33:2017-2025 (2019). Read more (PubMed: 30199284) »
