Overview

  • Product name
  • Description
    Rabbit polyclonal to RRM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-259 of RRM2 (P31350).

  • Positive control
    • 293T, A431, H1299, HeLa, Molt4 whole cell lysates; A549 cells; Human Cal27 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
  • Pathway
    Genetic information processing; DNA replication.
  • Sequence similarities
    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Post-translational
    modifications
    Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cb111 antibody
    • chunp6884 antibody
    • etID309896.20 antibody
    • R2 antibody
    • reductase M2 polypeptide variant 1 antibody
    • reductase M2 polypeptide variant 2 antibody
    • reductase M2 polypeptide variant 3a antibody
    • reductase M2 polypeptide variant 3b antibody
    • reductase M2 polypeptide variant 3c antibody
    • reductase M2 polypeptide variant 3d antibody
    • Ribonucleoside-diphosphate reductase subunit M2 antibody
    • Ribonucleotide reductase M2 antibody
    • Ribonucleotide reductase M2 polypeptide antibody
    • Ribonucleotide reductase M2 subunit antibody
    • ribonucleotide reductase protein r2 class I antibody
    • Ribonucleotide reductase regulatory subunit M2 antibody
    • Ribonucleotide reductase small chain antibody
    • Ribonucleotide reductase small subunit antibody
    • Ribonucleotide reductase, R2 subunit antibody
    • RIR2_HUMAN antibody
    • RR2 antibody
    • RR2M antibody
    • RRM2 antibody
    see all

Images

  • Anti-RRM2 antibody (ab154964) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling RRM2 with ab154964 at 1/500.
  • Immunofluorescence analysis of methanol fixed A549 cells labeling RRM2 with ab154964 at 1/500. Lower panel shows costaining with Hoechst 33342.

References

ab154964 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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