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    rrm2-antibody-ab154964.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
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Anti-RRM2 antibody (ab154964)

  • Datasheet
  • SDS
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Western blot - Anti-RRM2 antibody (ab154964)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)
  • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)

Key features and details

  • Rabbit polyclonal to RRM2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human RRM2 protein (ab99137)

View more associated products

Overview

  • Product name

    Anti-RRM2 antibody
    See all RRM2 primary antibodies
  • Description

    Rabbit polyclonal to RRM2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-259 of RRM2 (P31350).

  • Positive control

    • 293T, A431, H1299, HeLa, Molt4 whole cell lysates; A549 cells; Human Cal27 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human RRM2 protein (ab99137)
  • Related Products

    • Recombinant Human RRM2 protein (ab99137)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Cow
Zebrafish
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
  • Pathway

    Genetic information processing; DNA replication.
  • Sequence similarities

    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Post-translational
    modifications

    Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P31350 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6241 Human
    • Entrez Gene: 100330864 Zebrafish
    • Entrez Gene: 30733 Zebrafish
    • Omim: 180390 Human
    • SwissProt: P31350 Human
    • SwissProt: P79733 Zebrafish
    • Unigene: 226390 Human
    • Unigene: 75098 Zebrafish
    • Alternative names

      • cb111 antibody
      • chunp6884 antibody
      • etID309896.20 antibody
      • R2 antibody
      • reductase M2 polypeptide variant 1 antibody
      • reductase M2 polypeptide variant 2 antibody
      • reductase M2 polypeptide variant 3a antibody
      • reductase M2 polypeptide variant 3b antibody
      • reductase M2 polypeptide variant 3c antibody
      • reductase M2 polypeptide variant 3d antibody
      • Ribonucleoside-diphosphate reductase subunit M2 antibody
      • Ribonucleotide reductase M2 antibody
      • Ribonucleotide reductase M2 polypeptide antibody
      • Ribonucleotide reductase M2 subunit antibody
      • ribonucleotide reductase protein r2 class I antibody
      • Ribonucleotide reductase regulatory subunit M2 antibody
      • Ribonucleotide reductase small chain antibody
      • Ribonucleotide reductase small subunit antibody
      • Ribonucleotide reductase, R2 subunit antibody
      • RIR2_HUMAN antibody
      • RR2 antibody
      • RR2M antibody
      • RRM2 antibody
      see all

    Images

    • Western blot - Anti-RRM2 antibody (ab154964)
      Western blot - Anti-RRM2 antibody (ab154964)
      Anti-RRM2 antibody (ab154964) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 45 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody (ab154964)
      Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling RRM2 with ab154964 at 1/500.
    • Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)
      Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody (ab154964)
      Immunofluorescence analysis of methanol fixed A549 cells labeling RRM2 with ab154964 at 1/500. Lower panel shows costaining with Hoechst 33342.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab154964? Please let us know so that we can cite the reference in this datasheet.

    ab154964 has been referenced in 1 publication.

    • Hu CM  et al. Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation. FASEB J 33:2017-2025 (2019). PubMed: 30199284

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