Overview

  • Product name

    Anti-RRM2 antibody [EPR11820]
    See all RRM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11820] to RRM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RRM2. The exact sequence is proprietary.
    Database link: P31350

  • Positive control

    • HeLa, HepG2, K562, and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11820
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
    • Pathway

      Genetic information processing; DNA replication.
    • Sequence similarities

      Belongs to the ribonucleoside diphosphate reductase small chain family.
    • Post-translational
      modifications

      Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • cb111 antibody
      • chunp6884 antibody
      • etID309896.20 antibody
      • R2 antibody
      • reductase M2 polypeptide variant 1 antibody
      • reductase M2 polypeptide variant 2 antibody
      • reductase M2 polypeptide variant 3a antibody
      • reductase M2 polypeptide variant 3b antibody
      • reductase M2 polypeptide variant 3c antibody
      • reductase M2 polypeptide variant 3d antibody
      • Ribonucleoside-diphosphate reductase subunit M2 antibody
      • Ribonucleotide reductase M2 antibody
      • Ribonucleotide reductase M2 polypeptide antibody
      • Ribonucleotide reductase M2 subunit antibody
      • ribonucleotide reductase protein r2 class I antibody
      • Ribonucleotide reductase regulatory subunit M2 antibody
      • Ribonucleotide reductase small chain antibody
      • Ribonucleotide reductase small subunit antibody
      • Ribonucleotide reductase, R2 subunit antibody
      • RIR2_HUMAN antibody
      • RR2 antibody
      • RR2M antibody
      • RRM2 antibody
      see all

    Images

    • All lanes : Anti-RRM2 antibody [EPR11820] (ab172476) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa

    • Immunofluorescent analysis of HeLa cells labeling RRM2 with ab172476 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling RRM2 with ab172476 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    This product has been referenced in:

    • Qi W  et al. Inhibition of Wee1 sensitizes AML cells to ATR inhibitor VE-822-induced DNA damage and apoptosis. Biochem Pharmacol 164:273-282 (2019). Read more (PubMed: 31014753) »
    • Wang N  et al. Downregulation of ribonucleotide reductase subunits M2 induces apoptosis and G1 arrest of cervical cancer cells. Oncol Lett 15:3719-3725 (2018). Read more (PubMed: 29556274) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172476.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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