Overview

  • Product name
    Anti-RRM2 antibody [EPR11820]
    See all RRM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11820] to RRM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RRM2. The exact sequence is proprietary.
    Database link: P31350

  • Positive control
    • HeLa, HepG2, K562, and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11820
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
    • Pathway
      Genetic information processing; DNA replication.
    • Sequence similarities
      Belongs to the ribonucleoside diphosphate reductase small chain family.
    • Post-translational
      modifications
      Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • cb111 antibody
      • chunp6884 antibody
      • etID309896.20 antibody
      • R2 antibody
      • reductase M2 polypeptide variant 1 antibody
      • reductase M2 polypeptide variant 2 antibody
      • reductase M2 polypeptide variant 3a antibody
      • reductase M2 polypeptide variant 3b antibody
      • reductase M2 polypeptide variant 3c antibody
      • reductase M2 polypeptide variant 3d antibody
      • Ribonucleoside-diphosphate reductase subunit M2 antibody
      • Ribonucleotide reductase M2 antibody
      • Ribonucleotide reductase M2 polypeptide antibody
      • Ribonucleotide reductase M2 subunit antibody
      • ribonucleotide reductase protein r2 class I antibody
      • Ribonucleotide reductase regulatory subunit M2 antibody
      • Ribonucleotide reductase small chain antibody
      • Ribonucleotide reductase small subunit antibody
      • Ribonucleotide reductase, R2 subunit antibody
      • RIR2_HUMAN antibody
      • RR2 antibody
      • RR2M antibody
      • RRM2 antibody
      see all

    Images

    • All lanes : Anti-RRM2 antibody [EPR11820] (ab172476) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa

    • Immunofluorescent analysis of HeLa cells labeling RRM2 with ab172476 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling RRM2 with ab172476 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    This product has been referenced in:
    • Wang N  et al. Downregulation of ribonucleotide reductase subunits M2 induces apoptosis and G1 arrest of cervical cancer cells. Oncol Lett 15:3719-3725 (2018). Read more (PubMed: 29556274) »
    • Shinoda S  et al. Deferasirox, an oral iron chelator, with gemcitabine synergistically inhibits pancreatic cancer cell growth in vitro and in vivo. Oncotarget 9:28434-28444 (2018). Read more (PubMed: 29983871) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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