• Product name

  • Description

    Rabbit polyclonal to RRN3
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 601-651 of Human RRN3 (NP_060897.3 (GeneID 54700)).

  • Positive control

    • HeLa and Jurkat whole cell lysate (ab7899)



Our Abpromise guarantee covers the use of ab112052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
  • Sequence similarities

    Belongs to the RRN3 family.
  • Post-translational

    Phosphorylation is required for participation in rDNA transcription.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AL023001 antibody
    • DKFZp566E104 antibody
    • E130302O19Rik antibody
    • MGC104238 antibody
    • R75565 antibody
    • RGD1305001 antibody
    • RNA polymerase I-specific transcription initiation factor RRN3 antibody
    • Rrn3 antibody
    • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
    • RRN3_HUMAN antibody
    • TIF-IA antibody
    • TIF1A antibody
    • TIFIA antibody
    • Transcription Initiation Factor 1A antibody
    • Transcription initiation factor IA antibody
    • Transcription initiation factor TIF IA antibody
    see all


  • All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 74 kDa

    Exposure time: 3 minutes
  • ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 74 kDa Exposure time: 10 seconds


This product has been referenced in:

  • Wei T  et al. Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint. Cell Rep 23:404-414 (2018). WB . Read more (PubMed: 29642000) »
  • Von Walden F  et al. Muscle contractures in patients with cerebral palsy and acquired brain injury are associated with extracellular matrix expansion, pro-inflammatory gene expression, and reduced rRNA synthesis. Muscle Nerve 58:277-285 (2018). Read more (PubMed: 29572878) »
See all 5 Publications for this product

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