Product nameAnti-RRN3 antibody
See all RRN3 primary antibodies
DescriptionRabbit polyclonal to RRN3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between amino acids 601-651 of Human RRN3 (NP_060897.3 (GeneID 54700)).
- HeLa and Jurkat whole cell lysate (ab7899)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab112052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 74 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
Sequence similaritiesBelongs to the RRN3 family.
modificationsPhosphorylation is required for participation in rDNA transcription.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- AL023001 antibody
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All lanes : Anti-RRN3 antibody (ab112052) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes
ab112052 at 1 µg/ml staining RRN3 in HeLa whole cell lysate immunoprecipitated using ab112052 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: controle IgG. Predicted band size : 74 kDa Exposure time: 10 seconds
This product has been referenced in:
- Wei T et al. Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint. Cell Rep 23:404-414 (2018). WB . Read more (PubMed: 29642000) »
- Von Walden F et al. Muscle contractures in patients with cerebral palsy and acquired brain injury are associated with extracellular matrix expansion, pro-inflammatory gene expression, and reduced rRNA synthesis. Muscle Nerve 58:277-285 (2018). Read more (PubMed: 29572878) »