Anti-RRP4 antibody (ab156698)
Key features and details
- Rabbit polyclonal to RRP4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RRP4 antibody
See all RRP4 primary antibodies -
Description
Rabbit polyclonal to RRP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Opossum, Common marmoset, Orangutan, Xenopus tropicalis, Bat, Elephant -
Immunogen
Synthetic peptide corresponding to Human RRP4 aa 200-300.
Database link: Q13868 -
Positive control
- HeLa, Jurkat, NIH 3T3 and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab156698 was affinity purified using an epitope specific to EXOSC2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism. -
Cellular localization
Cytoplasmic, Exosome and Nuclear -
Database links
- Entrez Gene: 100397822 Common marmoset
- Entrez Gene: 615712 Cow
- Entrez Gene: 608606 Dog
- Entrez Gene: 101126796 Gorilla
- Entrez Gene: 23404 Human
- Entrez Gene: 227715 Mouse
- Entrez Gene: 100439017 Orangutan
- Entrez Gene: 595050 Xenopus tropicalis
see all -
Alternative names
- EXOSC2 antibody
- Exosome complex exonuclease RRP4 antibody
- Exosome component 2 antibody
see all
Images
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All lanes : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds -
All lanes : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml
Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes -
Detection of EXOSC2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118807 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab156698 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 : Alternative anti-RRP4 antibody
Lane 2 : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml
Lane 3 : Control IgG
All lanes : HEK293T whole cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab156698 has been referenced in 3 publications.
- Kikuchi K & Kaida D CCNE1 and E2F1 Partially Suppress G1 Phase Arrest Caused by Spliceostatin A Treatment. Int J Mol Sci 22:N/A (2021). PubMed: 34769053
- Yang X et al. Genetic and genomic studies of pathogenic EXOSC2 mutations in the newly described disease SHRF implicate the autophagy pathway in disease pathogenesis. Hum Mol Genet 29:541-553 (2020). PubMed: 31628467
- McIver SC et al. Exosome complex orchestrates developmental signaling to balance proliferation and differentiation during erythropoiesis. Elife 5:N/A (2016). IP . PubMed: 27543448