Overview

  • Product name

    Anti-RRP4 antibody [EPR13605] - N-terminal
    See all RRP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13605] to RRP4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RRP4 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q13868

  • Positive control

    • HepG2, HeLa and 293 cell lysates; Human kidney tissue; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Relevance

    The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
  • Cellular localization

    Cytoplasmic, Exosome and Nuclear
  • Database links

  • Alternative names

    • EXOSC2 antibody
    • Exosome complex exonuclease RRP4 antibody
    • Exosome component 2 antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4) antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease antibody
    • hRrp4p antibody
    • OTTHUMP00000022370 antibody
    • OTTHUMP00000022371 antibody
    • OTTHUMP00000022372 antibody
    • OTTHUMP00000022373 antibody
    • OTTHUMP00000022374 antibody
    • p7 antibody
    • Ribosomal RNA processing protein 4 antibody
    • RRP4 antibody
    • Rrp4p antibody
    see all

Images

  • Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181211 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling RRP4 with ab181211 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RRP4 with ab181211 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • All lanes : Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211) at 1/50000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 33 kDa

References

This product has been referenced in:

  • Davidson L  et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). Read more (PubMed: 30840897) »
See 1 Publication for this product

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