Product nameAnti-RRP4 antibody [EPR13606(B)] - C-terminal
See all RRP4 primary antibodies
DescriptionRabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RRP4 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q13868
- HepG2, HeLa, 293 and Jurkat cell lysates; Human stomach tissue; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceThe exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
Cellular localizationCytoplasmic, Exosome and Nuclear
- EXOSC2 antibody
- Exosome complex exonuclease RRP4 antibody
- Exosome component 2 antibody
All lanes : Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RRP4 with ab185235 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling RRP4 with ab185235 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
ab185235 has not yet been referenced specifically in any publications.