Recombinant
RabMAb

Recombinant Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)

Overview

  • Product name

    Anti-RRP4 antibody [EPR13606(B)] - C-terminal
    See all RRP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RRP4 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13868

  • Positive control

    • HepG2, HeLa, 293 and Jurkat cell lysates; Human stomach tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
  • Cellular localization

    Cytoplasmic, Exosome and Nuclear
  • Database links

  • Alternative names

    • EXOSC2 antibody
    • Exosome complex exonuclease RRP4 antibody
    • Exosome component 2 antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4) antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease antibody
    • hRrp4p antibody
    • OTTHUMP00000022370 antibody
    • OTTHUMP00000022371 antibody
    • OTTHUMP00000022372 antibody
    • OTTHUMP00000022373 antibody
    • OTTHUMP00000022374 antibody
    • p7 antibody
    • Ribosomal RNA processing protein 4 antibody
    • RRP4 antibody
    • Rrp4p antibody
    see all

Images

  • All lanes : Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RRP4 with ab185235 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling RRP4 with ab185235 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab185235 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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