Overview

  • Product name

    Anti-RRP42 antibody [EPR7452]
    See all RRP42 primary antibodies
  • Description

    Rabbit monoclonal [EPR7452] to RRP42
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RRP42 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat and NCI-H460 cell lysates; Human colon tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.90 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7452
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
    • Sequence similarities

      Belongs to the RNase PH family.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • EAP1 antibody
      • EXOS7_HUMAN antibody
      • Exosc7 antibody
      • Exosome complex component RRP42 antibody
      • Exosome complex exonuclease RRP42 antibody
      • Exosome component 7 antibody
      • FLJ26543 antibody
      • hRrp42p antibody
      • KIAA0116 antibody
      • p8 antibody
      • Ribosomal RNA processing protein 42 antibody
      • Ribosomal RNA-processing protein 42 antibody
      • RRP42 antibody
      • Rrp42p antibody
      see all

    Images

    • All lanes : Anti-RRP42 antibody [EPR7452] (ab126750) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : NCI-H460 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa

    • ab126750, at 1/100 dilution, staining RRP42 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126750 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (B-cell lymphocyte whole cell lysate)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Protein A
    Specification
    B-cell lymphocyte whole cell lysate

    Abcam user community

    Verified customer

    Submitted Mar 12 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    25 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 15 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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