Anti-RRS1 antibody (ab188161)
Key features and details
- Rabbit polyclonal to RRS1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RRS1 antibody -
Description
Rabbit polyclonal to RRS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human RRS1 aa 200-300.
Database link: Q15050 -
Positive control
- IHC-P: fallopian tube, pancreas, cerebral cortex and rectum tissue WB: PC-3 and HeLa cell lysate ICC/IF: U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa.
|
Notes |
---|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 41 kDa. |
Target
-
Function
Involved in ribosome biogenesis. -
Sequence similarities
Belongs to the RRS1 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 23212 Human
- Entrez Gene: 59014 Mouse
- Entrez Gene: 297784 Rat
- SwissProt: Q2KIH4 Cow
- SwissProt: Q15050 Human
- SwissProt: Q9CYH6 Mouse
- SwissProt: A1A5P2 Rat
- Unigene: 71827 Human
see all -
Alternative names
- Homolog of yeast ribosome biogenesis regulator antibody
- Homolog of yeast ribosome biogenesis regulatory protein RRS1 antibody
- KIAA0112 antibody
see all
Images
-
Immunohistochemical analysis of human rectum tissue labeling RRS1 with ab188161 at 1/1000 dilution.
-
Immunofluorescence analysis of Human cell line U-2 OS labeling RRS1 with ab188161 at 4 µg/ml.
-
All lanes : Anti-RRS1 antibody (ab188161) at 0.4 µg/ml
Lane 1 : PC-3 cell lysate
Lane 2 : HeLa cell lysate
Predicted band size: 41 kDa -
Immunohistochemical analysis of human fallopian tube tissue labeling RRS1 with ab188161 at 1/1000 dilution.
-
Immunohistochemical analysis of human pancreas tissue labeling RRS1 with ab188161 at 1/1000 dilution.
-
Immunohistochemical analysis of human cerebral cortex tissue labeling RRS1 with ab188161 at 1/1000 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab188161 has been referenced in 4 publications.
- Wang R et al. Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis. Int J Oncol 60:N/A (2022). PubMed: 35179222
- Ma Y et al. MicroRNA-598 inhibits the growth and maintenance of gastric cancer stem-like cells by down-regulating RRS1. Cell Cycle 18:2757-2769 (2019). PubMed: 31438772
- Wu XL et al. RRS1 silencing suppresses colorectal cancer cell proliferation and tumorigenesis by inhibiting G2/M progression and angiogenesis. Oncotarget 8:82968-82980 (2017). PubMed: 29137316
- Wang J et al. Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells. Oncol Rep 38:2166-2172 (2017). PubMed: 28849112