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Do you know the using concentration for blocking CCR2 in an in vitro culture of monocytes?
Asked on Sep 25 2013
The amount of product required depends on many factors including target accessibility, cell permeability, duration of incubation, and type of cells or assay used, it is best to survey the literature to determine the IC50, EC50 or Ki. For an inhibitor, if published Ki or, IC50 values are known, we recommend that you use 5 to 10 times higher than these values to maximally inhibit enzyme or receptor activity. If there are no published values we recommend that you perform dose-response experiments (running appropriate controls) and use Michaelis-Menten kinetics to determine the Ki value.
From my quick research, I found that for the pharmacological inhibition of CCR2, the specific CCR2 antagonist RS 504393 has a IC50 for CCR2 of 98 nM. RS 504393 inhibits the MCP-1-induced chemotaxis with an IC50 of 330 nM.
Elisa ThomasAbcam Scientific Support
Answered on Sep 25 2013