Product nameAnti-rSec6 antibody [EPR10812]
See all rSec6 primary antibodies
DescriptionRabbit monoclonal [EPR10812] to rSec6
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human rSec6. The exact sequence is proprietary.
Database link: O60645
- HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab156568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
FunctionComponent of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Sequence similaritiesBelongs to the SEC6 family.
- Information by UniProt
- EXOC 3 antibody
- Exoc3 antibody
- EXOC3_HUMAN antibody
All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF7 lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 87 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.
Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.