Overview

  • Product name
    Anti-rSec6 antibody [EPR10812]
    See all rSec6 primary antibodies
  • Description
    Rabbit monoclonal [EPR10812] to rSec6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human rSec6. The exact sequence is proprietary.
    Database link: O60645

  • Positive control
    • HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR10812
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : MCF7 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 87 kDa
      Observed band size: 85 kDa
      why is the actual band size different from the predicted?

    • Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.
    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.
    • Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.

    References

    This product has been referenced in:
    • Tanaka T  et al. Sec6 enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38 MAPK, MK2, and HSP27. Cell Signal 49:1-16 (2018). WB, IP, ICC/IF . Read more (PubMed: 29729335) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary culture hippocampus cell)
    Gel Running Conditions
    Non-reduced Denaturing (15% SDS-PAGE)
    Loading amount
    15 µg
    Treatment
    RIPA & 1%Protease inhibitor for 15min on ice
    Specification
    primary culture hippocampus cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 08 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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