RecombinantRabMAb

Recombinant Anti-rSec6 antibody [EPR10812] (ab156568)

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Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)
  • Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
  • Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)
  • Anti-rSec6 antibody [EPR10812] (ab156568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10812] to rSec6
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-rSec6 antibody [EPR10812]
    See all rSec6 primary antibodies

  • Description

    Rabbit monoclonal [EPR10812] to rSec6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human rSec6. The exact sequence is proprietary.
    Database link: O60645

  • Positive control

    • HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10812

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)
      Western blot - Anti-rSec6 antibody [EPR10812] (ab156568)
      All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : MCF7 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 87 kDa
      Observed band size: 85 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunoprecipitation - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunocytochemistry/ Immunofluorescence - Anti-rSec6 antibody [EPR10812] (ab156568)
      Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.
    • Anti-rSec6 antibody [EPR10812] (ab156568)
      Anti-rSec6 antibody [EPR10812] (ab156568)

    References (1)

    Publishing research using ab156568? Please let us know so that we can cite the reference in this datasheet.

    ab156568 has been referenced in 1 publication.

    • Tanaka T  et al. Sec6 enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38 MAPK, MK2, and HSP27. Cell Signal 49:1-16 (2018). WB, IP, ICC/IF . PubMed: 29729335

    Customer reviews and Q&As

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    Western blot abreview for Anti-rSec6 antibody [EPR10812]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary culture hippocampus cell)
    Gel Running Conditions
    Non-reduced Denaturing (15% SDS-PAGE)
    Loading amount
    15 µg
    Treatment
    RIPA & 1%Protease inhibitor for 15min on ice
    Specification
    primary culture hippocampus cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    Abcam user community

    Verified customer

    Submitted Oct 08 2018

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