Recombinant Anti-rSec6 antibody [EPR10812] (ab156568)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10812] to rSec6
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-rSec6 antibody [EPR10812]
See all rSec6 primary antibodies -
Description
Rabbit monoclonal [EPR10812] to rSec6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human rSec6. The exact sequence is proprietary.
Database link: O60645 -
Positive control
- HeLa, MCF7, HepG2, and fetal brain lysates, Human kidney and Human pancreas tissues, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10812 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. -
Sequence similarities
Belongs to the SEC6 family. - Information by UniProt
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Database links
- Entrez Gene: 11336 Human
- Entrez Gene: 211446 Mouse
- Entrez Gene: 252881 Rat
- Omim: 608186 Human
- SwissProt: O60645 Human
- SwissProt: Q6KAR6 Mouse
- SwissProt: Q62825 Rat
- Unigene: 481464 Human
see all -
Alternative names
- EXOC 3 antibody
- Exoc3 antibody
- EXOC3_HUMAN antibody
see all
Images
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All lanes : Anti-rSec6 antibody [EPR10812] (ab156568) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF7 lysate
Lane 3 : HepG2 lysate
Lane 4 : fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 87 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted? -
Immunoprecipitation. ab156568 at 1/1000 staining rSec6 in MCF7 lysate immunoprecipitated using ab156568 at 1/10. Predicted band size : 85 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling rSec6 with ab156568 antibody at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-rSec6 antibody [EPR10812] (ab156568)
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling rSec6 with ab156568 antibody at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling rSec6 with ab156568 at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab156568 has been referenced in 1 publication.
- Tanaka T et al. Sec6 enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38 MAPK, MK2, and HSP27. Cell Signal 49:1-16 (2018). WB, IP, ICC/IF . PubMed: 29729335