Overview

  • Product name
    Anti-RSF1 antibody [EPR3749(2)]
    See all RSF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3749(2)] to RSF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human RSF1.

  • Positive control
    • WB: Jurkat, HEK-293T and HeLa cell lysates. IHC-P: Human colon, endometrial adenocarcinoma and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

For unpurified use at 1/500-1/1000.

WB 1/1000 - 1/10000. Predicted molecular weight: 164 kDa.
IHC-P 1/1000.

Antigen retrieval is recommended. See IHC antigen retrieval protocols.

For unpurified use at 1/250- 1/500.

 

Flow Cyt 1/80.

Target

  • Relevance
    RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • HBV pX associated protein 8 antibody
    • Hepatitis B virus x associated protein antibody
    • HXBAP antibody
    • p325 antibody
    • p325 subunit of RSF chromatin remodeling complex antibody
    • remodeling and spacing factor 1 antibody
    • RSF 1 antibody
    • XAP8 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1:100 (8.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • All lanes : Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 164 kDa

  • Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Min S  et al. Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage. Mol Cells 41:127-133 (2018). WB . Read more (PubMed: 29385673) »
  • Lee HS  et al. The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nat Commun 6:7904 (2015). ICC . Read more (PubMed: 26259146) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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