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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

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Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
  • Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
  • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
  • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein (ab172182)

View more associated products

Overview

  • Product name

    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
  • Description

    Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for human Rsk 2 / MAPKAP Kinase 1b.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (C terminal). The exact sequence is proprietary.

  • Epitope

    ab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b.
  • Positive control

    • MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y82
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Associated products

  • Alternative Versions

    • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] - BSA and Azide free (ab247218)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Rsk 2 / MAPKAP Kinase 1b (mutated L608F) protein (ab172182)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 90 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000. Predicted molecular weight: 90 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Tissue specificity

      Expressed in many tissues, highest levels in skeletal muscle.
    • Involvement in disease

      Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Autophosphorylated on Ser-386, as part of the activation process.
      Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
    • Target information above from: UniProt accession P51812 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6197 Human
      • Entrez Gene: 110651 Mouse
      • Entrez Gene: 501560 Rat
      • Omim: 300075 Human
      • SwissProt: P51812 Human
      • SwissProt: P18654 Mouse
      • Unigene: 445387 Human
      • Unigene: 328476 Mouse
      • Unigene: 204789 Rat
      see all
    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 3 antibody
      • CLS antibody
      • HU 3 antibody
      • HU2 antibody
      • HU3 antibody
      • Insulin stimulated protein kinase 1 antibody
      • Insulin-stimulated protein kinase 1 antibody
      • ISPK-1 antibody
      • ISPK1 antibody
      • KS6A3_HUMAN antibody
      • MAP kinase activated protein kinase 1b antibody
      • MAP kinase-activated protein kinase 1b antibody
      • MAPK activated protein kinase 1b antibody
      • MAPK-activated protein kinase 1b antibody
      • MAPKAP kinase 1b antibody
      • MAPKAPK 1b antibody
      • MAPKAPK-1b antibody
      • MAPKAPK1B antibody
      • Mental retardation, X linked 19 antibody
      • MRX19 antibody
      • OTTHUMP00000023036 antibody
      • p90 RSK2 antibody
      • p90 RSK3 antibody
      • p90-RSK 3 antibody
      • p90RSK3 antibody
      • pp90RSK2 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
      • Ribosomal protein S6 kinase alpha 3 antibody
      • Ribosomal protein S6 kinase alpha-3 antibody
      • Ribosomal protein s6 kinase ii alpha 2 antibody
      • Ribosomal S6 kinase 2 antibody
      • Rps6ka3 antibody
      • RSK antibody
      • RSK-2 antibody
      • RSK2 antibody
      • S6 kinase 2 antibody
      • S6K alpha3 antibody
      • S6K-alpha-3 antibody
      see all

    Images

    • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
      Lane 3: MCF7 cell lysate (40 µg)
      Lane 4: HeLa cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

      ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
      Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab32062? Please let us know so that we can cite the reference in this datasheet.

    ab32062 has not yet been referenced specifically in any publications.

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