Recombinant Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-269785-anti-rsk-2-mapkap-kinase-1b-antibody-y82-western-blot.jpg "Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies -
Description
Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b -
Host species
Rabbit -
Specificity
This antibody is specific for human Rsk 2 / MAPKAP Kinase 1b. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (C terminal). The exact sequence is proprietary.
-
Epitope
ab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b. -
Positive control
- MCF7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y82 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 90 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 90 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Tissue specificity
Expressed in many tissues, highest levels in skeletal muscle. -
Involvement in disease
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
-
Database links
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
Images
-
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: HeLa cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-43776-anti-rsk-2-mapkap-kinase-1b-antibody-y82-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-215888-anti-rsk-2-mapkap-kinase-1b-antibody-y82-flow-cytometry.jpg)
Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-43777-anti-rsk-2-mapkap-kinase-1b-antibody-y82-western-blot.jpg)
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate.
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-5-benefits-of-recombinant-antibodies.png)
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab32062 has not yet been referenced specifically in any publications.
