Recombinant Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies -
DescriptionRabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
-
Host speciesRabbit
-
SpecificityThis antibody is specific for human Rsk 2 / MAPKAP Kinase 1b.
-
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IPmore details
Unsuitable for: ICC -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (C terminal). The exact sequence is proprietary.
-
Epitopeab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b.
-
Positive control
- MCF7 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberY82
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 90 kDa. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IP | 1/50. |
Target
-
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
-
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
-
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
-
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
-
Database links
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
Images
-
![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-269785-ab32062KOWB.jpg)
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: HeLa cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue. -
![Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062-3-ab32062FC.jpg)
Flow Cytometry - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)](https://www.abcam.com/ps/products/32/ab32062/Images/ab32062_1.jpg)
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062)Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Datasheets and documents
References
ab32062 has not yet been referenced specifically in any publications.
