Product nameAnti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
DescriptionRabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
SpecificityThe antibody recognises Rsk2. It does not cross react with other S6 kinase family members.
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IP, ChIPmore details
Unsuitable for: ICC
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to residues near N-terminus of human RSK2
- HeLa whole cell lysate (ab150035) and human lymphoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ChIP||Use at an assay dependent concentration. PubMed: 25368387|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773
andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + Hela cell lysate
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Takada I DGCR14 induces Il17a gene expression through the ROR?/BAZ1B/RSKS2 complex. Mol Cell Biol 35:344-55 (2015). WB, ChIP ; Mouse . Read more (PubMed: 25368387) »
- Yu G et al. RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Mol Cancer Res 13:348-57 (2015). Read more (PubMed: 25189355) »