Product nameRsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell)
ab94181 is a 293T cell transfected lysate in which Human Rsk 2 / MAPKAP Kinase 1b has been transiently over-expressed using a pCMV-Rsk 2 / MAPKAP Kinase 1b plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Tissue specificity: Expressed in many tissues, highest levels in skeletal muscle. Disease: Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains. PTM: Autophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
Our Abpromise guarantee covers the use of ab94181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94181 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b antibody (ab76886) at 1/500 dilution
Lane 1 :
Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94181 has not yet been referenced specifically in any publications.