Key features and details
- Rabbit polyclonal to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
DescriptionRabbit polyclonal to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Xenopus laevis
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (internal sequence) (phospho S227). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P51812
- WB: NIH/3T3 whole cell extract.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 84 kDa.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody (ab226921) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 84 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226921 has not yet been referenced specifically in any publications.