Product nameAnti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
DescriptionRabbit polyclonal to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Xenopus laevis
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (internal sequence) (phospho S227). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P51812
- WB: NIH/3T3 whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 84 kDa.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody (ab226921) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 84 kDa
10% SDS-PAGE gel.
ab226921 has not yet been referenced specifically in any publications.