Overview

  • Product name

    Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
  • Description

    Rabbit polyclonal to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (internal sequence) (phospho S227). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P51812

  • Positive control

    • WB: NIH/3T3 whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.

Target

  • Function

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Tissue specificity

    Expressed in many tissues, highest levels in skeletal muscle.
  • Involvement in disease

    Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications

    Autophosphorylated on Ser-386, as part of the activation process.
    Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
  • Information by UniProt
  • Database links

  • Alternative names

    • 90 kDa ribosomal protein S6 kinase 3 antibody
    • CLS antibody
    • HU 3 antibody
    • HU2 antibody
    • HU3 antibody
    • Insulin stimulated protein kinase 1 antibody
    • Insulin-stimulated protein kinase 1 antibody
    • ISPK-1 antibody
    • ISPK1 antibody
    • KS6A3_HUMAN antibody
    • MAP kinase activated protein kinase 1b antibody
    • MAP kinase-activated protein kinase 1b antibody
    • MAPK activated protein kinase 1b antibody
    • MAPK-activated protein kinase 1b antibody
    • MAPKAP kinase 1b antibody
    • MAPKAPK 1b antibody
    • MAPKAPK-1b antibody
    • MAPKAPK1B antibody
    • Mental retardation, X linked 19 antibody
    • MRX19 antibody
    • OTTHUMP00000023036 antibody
    • p90 RSK2 antibody
    • p90 RSK3 antibody
    • p90-RSK 3 antibody
    • p90RSK3 antibody
    • pp90RSK2 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
    • Ribosomal protein S6 kinase alpha 3 antibody
    • Ribosomal protein S6 kinase alpha-3 antibody
    • Ribosomal protein s6 kinase ii alpha 2 antibody
    • Ribosomal S6 kinase 2 antibody
    • Rps6ka3 antibody
    • RSK antibody
    • RSK-2 antibody
    • RSK2 antibody
    • S6 kinase 2 antibody
    • S6K alpha3 antibody
    • S6K-alpha-3 antibody
    see all

Images

  • All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody (ab226921) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast cell line) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 84 kDa



    10% SDS-PAGE gel.

References

ab226921 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226921.
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