Recombinant
RabMAb

Recombinant Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820)

Overview

  • Product name

    Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y]
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
  • Description

    Rabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
  • Host species

    Rabbit
  • Specificity

    ab75820 only detects Rsk 2 / MAPKAP Kinase 1b phosphorylated on Serine 227
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 227 of human Rsk 2/ MAPKAP Kinase 1b

  • Positive control

    • HeLa whole cell lysate (ab150035), untreated or treated with calyculin A.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2847Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Tissue specificity

      Expressed in many tissues, highest levels in skeletal muscle.
    • Involvement in disease

      Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Autophosphorylated on Ser-386, as part of the activation process.
      Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
    • Information by UniProt
    • Database links

    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 3 antibody
      • CLS antibody
      • HU 3 antibody
      • HU2 antibody
      • HU3 antibody
      • Insulin stimulated protein kinase 1 antibody
      • Insulin-stimulated protein kinase 1 antibody
      • ISPK-1 antibody
      • ISPK1 antibody
      • KS6A3_HUMAN antibody
      • MAP kinase activated protein kinase 1b antibody
      • MAP kinase-activated protein kinase 1b antibody
      • MAPK activated protein kinase 1b antibody
      • MAPK-activated protein kinase 1b antibody
      • MAPKAP kinase 1b antibody
      • MAPKAPK 1b antibody
      • MAPKAPK-1b antibody
      • MAPKAPK1B antibody
      • Mental retardation, X linked 19 antibody
      • MRX19 antibody
      • OTTHUMP00000023036 antibody
      • p90 RSK2 antibody
      • p90 RSK3 antibody
      • p90-RSK 3 antibody
      • p90RSK3 antibody
      • pp90RSK2 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
      • Ribosomal protein S6 kinase alpha 3 antibody
      • Ribosomal protein S6 kinase alpha-3 antibody
      • Ribosomal protein s6 kinase ii alpha 2 antibody
      • Ribosomal S6 kinase 2 antibody
      • Rps6ka3 antibody
      • RSK antibody
      • RSK-2 antibody
      • RSK2 antibody
      • S6 kinase 2 antibody
      • S6K alpha3 antibody
      • S6K-alpha-3 antibody
      see all

    Images

    • All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820) at 1/2000 dilution

      Lane 1 : HeLa cell
      lysates, untreated
      Lane 2 : HeLa cell
      lysates, treated
      with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa

    References

    ab75820 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab75820.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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