Product nameAnti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
DescriptionRabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227)
Specificityab75820 only detects Rsk 2 / MAPKAP Kinase 1b phosphorylated on Serine 227
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
A phospho-specific peptide corresponding to residues surrounding Serine 227 of human Rsk 2/ MAPKAP Kinase 1b
- HeLa whole cell lysate (ab150035), untreated or treated with calyculin A.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820) at 1/2000 dilution
Lane 1 : HeLa cell
Lane 2 : HeLa cell
with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
ab75820 has not yet been referenced specifically in any publications.