Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] - BSA and Azide free
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
DescriptionRabbit monoclonal [EPR2847Y] to Rsk 2 / MAPKAP Kinase 1b (phospho S227) - BSA and Azide free
This antibody only detects Rsk 2 / MAPKAP Kinase 1b phosphorylated on Serine 227.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (phospho S227). The exact sequence is proprietary.
Ab247405 is the carrier-free version of ab75820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247405 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab247405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b (phospho S227) antibody [EPR2847Y] (ab75820) at 1/2000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
This data was developed using ab75820, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247405 has not yet been referenced specifically in any publications.