Product nameAnti-RSK1 p90 antibody [E4]
See all RSK1 p90 primary antibodies
DescriptionRabbit monoclonal [E4] to RSK1 p90
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Synthetic peptide within Human RSK1 p90 (N terminal). The exact sequence is proprietary.
- WB: MCF7 cell lysate. IHC-P: Human colon tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-380, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RSK1 p90 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32114 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32114 was shown to recognize RSK1 p90 when RSK1 p90 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 p90 knockout samples were subjected to SDS-PAGE. ab32114 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Ab32114, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human colon tissue by immunohistochemistry.
Overlay histogram showing HeLa cells stained with ab32114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32114, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-RSK1 p90 antibody [E4] (ab32114) at 1/1000 dilution + MCF7 cell lysate at 10 µg
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Su L et al. Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway. Exp Ther Med 15:4185-4190 (2018). Read more (PubMed: 29731817) »
- Fu S et al. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity. Sci Rep 7:43771 (2017). Read more (PubMed: 28256562) »