Overview

  • Product name
    Anti-RSK1 p90 antibody [E4]
    See all RSK1 p90 primary antibodies
  • Description
    Rabbit monoclonal [E4] to RSK1 p90
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human RSK1 p90 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: MCF7 cell lysate. IHC-P: Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/200.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10.

Target

  • Function
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Autophosphorylated on Ser-380, as part of the activation process.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 1 antibody
    • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
    • dJ590P13.1 antibody
    • EC 2.7.11.1 antibody
    • HU 1 antibody
    • HU1 antibody
    • KS6A1_HUMAN antibody
    • MAP kinase activated protein kinase 1a antibody
    • MAP kinase-activated protein kinase 1a antibody
    • MAPK-activated protein kinase 1a antibody
    • MAPKAP kinase 1a antibody
    • MAPKAPK-1a antibody
    • MAPKAPK1A antibody
    • MGC79981 antibody
    • Mitogen-activated protein kinase-activated protein kinase 1A antibody
    • OTTHUMP00000004113 antibody
    • p90 RSK1 antibody
    • p90-RSK 1 antibody
    • p90rsk antibody
    • p90RSK1 antibody
    • p90S6K antibody
    • pp90RSK1 antibody
    • Ribosomal protein S6 kinase 90kD 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase alpha 1 antibody
    • Ribosomal protein S6 kinase alpha-1 antibody
    • Ribosomal protein S6 kinase polypeptide 1 antibody
    • Ribosomal S6 kinase 1 antibody
    • RPS6K1 alpha antibody
    • rps6ka antibody
    • Rps6ka1 antibody
    • RSK 1 antibody
    • RSK 1 p90 antibody
    • RSK antibody
    • RSK-1 antibody
    • RSK1 antibody
    • S6K alpha 1 antibody
    • S6K-alpha-1 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RSK1 p90 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32114 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab32114 was shown to recognize RSK1 p90 when RSK1 p90 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 p90 knockout samples were subjected to SDS-PAGE. ab32114 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Ab32114, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human colon tissue by immunohistochemistry.
  • Overlay histogram showing HeLa cells stained with ab32114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32114, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-RSK1 p90 antibody [E4] (ab32114) at 1/1000 dilution + MCF7 cell lysate at 10 µg

    Predicted band size: 83 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Su L  et al. Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway. Exp Ther Med 15:4185-4190 (2018). Read more (PubMed: 29731817) »
  • Fu S  et al. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity. Sci Rep 7:43771 (2017). Read more (PubMed: 28256562) »
See all 5 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Human Tissue sections (Human fibromatosis)
Specification
Human fibromatosis
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (mouse liver)
Specification
mouse liver
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 25 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Pig Cell lysate - whole cell (PAE cells)
Specification
PAE cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 12 2014

Answer

Thank you for your enquiry. Based on sequence analysis of the immunogen, ab32114 RSK1 p90 antibody [E4] would not cross react with other RSK proteins RSK2, RSK3 or RSK4. However, I am sorry this has not been been verified by testing in the laboratory on this occasion. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cell line)
Loading amount
10000 cells
Specification
THP-1 cell line
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 07 2008

Answer

Thank you for your phone call. I now have the information for you. The current concentration of this antibody is 360ug/ml, from cell supernatant. I hope this helps. Please get back in touch if you have any more questions, and I will be happy to help. With thanks,

Read More

Question
Answer

Thank you for your phone call. I now have the information for you. The current concentration of this antibody is 360ug/ml, from cell supernatant. I hope this helps. Please get back in touch if you have any more questions, and I will be happy to help. With thanks,

Read More

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