Overview

  • Product name

    Anti-RSK1 p90 antibody [E4] - BSA and Azide free
    See all RSK1 p90 primary antibodies
  • Description

    Rabbit monoclonal [E4] to RSK1 p90 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide within Human RSK1 p90 (N terminal). The exact sequence is proprietary.

  • General notes

    Ab239805 is the carrier-free version of ab32114. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239805 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications

    Autophosphorylated on Ser-380, as part of the activation process.
  • Information by UniProt
  • Database links

  • Alternative names

    • 90 kDa ribosomal protein S6 kinase 1 antibody
    • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
    • dJ590P13.1 antibody
    • EC 2.7.11.1 antibody
    • HU 1 antibody
    • HU1 antibody
    • KS6A1_HUMAN antibody
    • MAP kinase activated protein kinase 1a antibody
    • MAP kinase-activated protein kinase 1a antibody
    • MAPK-activated protein kinase 1a antibody
    • MAPKAP kinase 1a antibody
    • MAPKAPK-1a antibody
    • MAPKAPK1A antibody
    • MGC79981 antibody
    • Mitogen-activated protein kinase-activated protein kinase 1A antibody
    • OTTHUMP00000004113 antibody
    • p90 RSK1 antibody
    • p90-RSK 1 antibody
    • p90rsk antibody
    • p90RSK1 antibody
    • p90S6K antibody
    • pp90RSK1 antibody
    • Ribosomal protein S6 kinase 90kD 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase alpha 1 antibody
    • Ribosomal protein S6 kinase alpha-1 antibody
    • Ribosomal protein S6 kinase polypeptide 1 antibody
    • Ribosomal S6 kinase 1 antibody
    • RPS6K1 alpha antibody
    • rps6ka antibody
    • Rps6ka1 antibody
    • RSK 1 antibody
    • RSK 1 p90 antibody
    • RSK antibody
    • RSK-1 antibody
    • RSK1 antibody
    • RSK1p90 antibody
    • S6K alpha 1 antibody
    • S6K-alpha-1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling RSK1 p90 with purified ab32114 at 1/100 dilution (15.4 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).

  • Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling RSK1 p90 with purified ab32114 at 1:150 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).
  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSK1 p90 with purified ab32114 at 1/150 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).

  • ab32114 (purified) at 1/70 dilution (2ug) immunoprecipitating RSK1 p90 in K-562 whole cell lysate. K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug
    Lane 2 (+): ab32114 & K-562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32114 in K-562 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).

  • Overlay histogram showing HeLa cells stained with unpurified ab32114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32114, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).

  • Ab32114 (unpurified), at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human colon tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32114).

References

ab239805 has not yet been referenced specifically in any publications.

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