Product nameAnti-RSK1 p90 antibody [Y81]
See all RSK1 p90 primary antibodies
DescriptionRabbit monoclonal [Y81] to RSK1 p90
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RSK1 p90 (C terminal). The exact sequence is proprietary.
- MCF7 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-380, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RSK1 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF-7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution + MCF7 cell lysate
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Wang X et al. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway. Cell Death Dis 9:578 (2018). Read more (PubMed: 29784961) »
- Lu W et al. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling. Sci Rep 7:8402 (2017). Read more (PubMed: 28827748) »