Overview

  • Product name
    Anti-RSK1 p90 (phospho S221) antibody
    See all RSK1 p90 primary antibodies
  • Description
    Rabbit polyclonal to RSK1 p90 (phospho S221)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human).

  • Positive control
    • A431 and NIH3T3 cells treated with PMA, a phorbol ester activator of protein kinase C.

Properties

Applications

Our Abpromise guarantee covers the use of ab10695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 90 kDa.

Target

  • Function
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications
    Autophosphorylated on Ser-380, as part of the activation process.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa ribosomal protein S6 kinase 1 antibody
    • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
    • dJ590P13.1 antibody
    • EC 2.7.11.1 antibody
    • HU 1 antibody
    • HU1 antibody
    • KS6A1_HUMAN antibody
    • MAP kinase activated protein kinase 1a antibody
    • MAP kinase-activated protein kinase 1a antibody
    • MAPK-activated protein kinase 1a antibody
    • MAPKAP kinase 1a antibody
    • MAPKAPK-1a antibody
    • MAPKAPK1A antibody
    • MGC79981 antibody
    • Mitogen-activated protein kinase-activated protein kinase 1A antibody
    • OTTHUMP00000004113 antibody
    • p90 RSK1 antibody
    • p90-RSK 1 antibody
    • p90rsk antibody
    • p90RSK1 antibody
    • p90S6K antibody
    • pp90RSK1 antibody
    • Ribosomal protein S6 kinase 90kD 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase alpha 1 antibody
    • Ribosomal protein S6 kinase alpha-1 antibody
    • Ribosomal protein S6 kinase polypeptide 1 antibody
    • Ribosomal S6 kinase 1 antibody
    • RPS6K1 alpha antibody
    • rps6ka antibody
    • Rps6ka1 antibody
    • RSK 1 antibody
    • RSK 1 p90 antibody
    • RSK antibody
    • RSK-1 antibody
    • RSK1 antibody
    • S6K alpha 1 antibody
    • S6K-alpha-1 antibody
    see all

Images

  • Peptide Competition - Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% non-fat dried milk-TBST buffer for one hour at room temperature and incubated with ab10695 for one hour at room temperature in a 1% non-fat dried milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 1% non-fat dried milk-TBST buffer, and bands were detected by chemiluminescence. The data show induction of ab10695 signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Co

References

This product has been referenced in:
  • Deng L  et al. Luteolin, a novel p90 ribosomal S6 kinase inhibitor, suppresses proliferation and migration in leukemia cells. Oncol Lett 13:1370-1378 (2017). WB ; Human . Read more (PubMed: 28454264) »
  • Duan RB  et al. Two p90 ribosomal S6 kinase isoforms are involved in the regulation of mitotic and meiotic arrest in Artemia. J Biol Chem 289:16006-15 (2014). Read more (PubMed: 24755224) »
See all 7 Publications for this product

Customer reviews and Q&As

Question
Answer

I am very pleased to inform you I have the information you need at last. We do not put the concentration on the datasheet as the source' s packages are based on volume, in order to be able to adjust the concentration of antibody to assure consistent batch-to-batch signal strength using our prequalified test systems (in WB, the only tested application). Therefore the actual amount of antibody was felt to be less critical than the signal strength provided. However, as you are using the antibody for applications other than WB we will give you the concentration. The concentration of ab10695 is 0.5mg/ml for lots number 79388, 74910, 81069, 77894, 75191, 79680. According to your order you had lot 81069. Please could you let me know how you get on with your FACS experiments? If do we will send you an Amazon gift voucher worth £15. Best of luck with your studies,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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