Anti-RSK1 p90 (phospho S732) antibody (ab106087)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RSK1 p90 (phospho S732) antibody
See all RSK1 p90 primary antibodies -
Description
Rabbit polyclonal to RSK1 p90 (phospho S732) -
Host species
Rabbit -
Specificity
ab106087 recognises RSK1 p90 only when phosphorilated at Ser 732. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Opossum
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Immunogen
A synthetic phosphopeptide corresponding to a region surrounding phospho S732 at the C-terminal end of Human RSK1 p90.
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Positive control
- EGF stimulated HEK293T cell lysates.
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General notes
Expiration date is one (1) year from date of opening product. Dilute only prior to immediate use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106087 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 83 kDa. |
Target
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Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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Database links
- Entrez Gene: 533908 Cow
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Images
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Western blot - Anti-RSK1 p90 (phospho S732) antibody (ab106087)All lanes : Anti-RSK1 p90 (phospho S732) antibody (ab106087) at 1/1000 dilution
Lane 1 : unstimulated HEK293T cell lysates (absence of serum for 12 h)
Lane 2 : EGF stimulated (lane 2) HEK293T cell lysates (absence of serum for 12 h)
Secondary
All lanes : Goat-anti-Rabbit HRP
Developed using the ECL technique.
Predicted band size: 83 kDa
Protocols
References
ab106087 has not yet been referenced specifically in any publications.
