Anti-RSK1 p90 (phospho S732) antibody (ab106087)
Key features and details
- Rabbit polyclonal to RSK1 p90 (phospho S732)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RSK1 p90 (phospho S732) antibody
See all RSK1 p90 primary antibodies -
Description
Rabbit polyclonal to RSK1 p90 (phospho S732) -
Host species
Rabbit -
Specificity
ab106087 recognises RSK1 p90 only when phosphorilated at Ser 732. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Opossum
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Immunogen
A synthetic phosphopeptide corresponding to a region surrounding phospho S732 at the C-terminal end of Human RSK1 p90.
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Positive control
- EGF stimulated HEK293T cell lysates.
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General notes
Expiration date is one (1) year from date of opening product. Dilute only prior to immediate use.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106087 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 83 kDa. |
Target
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Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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Database links
- Entrez Gene: 533908 Cow
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Images
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Western blot - Anti-RSK1 p90 (phospho S732) antibody (ab106087)All lanes : Anti-RSK1 p90 (phospho S732) antibody (ab106087) at 1/1000 dilution
Lane 1 : unstimulated HEK293T cell lysates (absence of serum for 12 h)
Lane 2 : EGF stimulated (lane 2) HEK293T cell lysates (absence of serum for 12 h)
Secondary
All lanes : Goat-anti-Rabbit HRP
Developed using the ECL technique.
Predicted band size: 83 kDa
References (0)
ab106087 has not yet been referenced specifically in any publications.
