Overview

  • Product name

    Anti-RSK1 p90 (phospho T348) antibody
    See all RSK1 p90 primary antibodies
  • Description

    Rabbit polyclonal to RSK1 p90 (phospho T348)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho peptide conjugated to KLH from around the phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from Human RSK1 p90 (NP_001006666.1).

  • Positive control

    • Methanol fixed HeLa cells; HeLa cell extracts (UV treated); Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131350 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • Post-translational
    modifications

    Autophosphorylated on Ser-380, as part of the activation process.
  • Information by UniProt
  • Database links

  • Alternative names

    • 90 kDa ribosomal protein S6 kinase 1 antibody
    • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
    • dJ590P13.1 antibody
    • EC 2.7.11.1 antibody
    • HU 1 antibody
    • HU1 antibody
    • KS6A1_HUMAN antibody
    • MAP kinase activated protein kinase 1a antibody
    • MAP kinase-activated protein kinase 1a antibody
    • MAPK-activated protein kinase 1a antibody
    • MAPKAP kinase 1a antibody
    • MAPKAPK-1a antibody
    • MAPKAPK1A antibody
    • MGC79981 antibody
    • Mitogen-activated protein kinase-activated protein kinase 1A antibody
    • OTTHUMP00000004113 antibody
    • p90 RSK1 antibody
    • p90-RSK 1 antibody
    • p90rsk antibody
    • p90RSK1 antibody
    • p90S6K antibody
    • pp90RSK1 antibody
    • Ribosomal protein S6 kinase 90kD 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase alpha 1 antibody
    • Ribosomal protein S6 kinase alpha-1 antibody
    • Ribosomal protein S6 kinase polypeptide 1 antibody
    • Ribosomal S6 kinase 1 antibody
    • RPS6K1 alpha antibody
    • rps6ka antibody
    • Rps6ka1 antibody
    • RSK 1 antibody
    • RSK 1 p90 antibody
    • RSK antibody
    • RSK-1 antibody
    • RSK1 antibody
    • S6K alpha 1 antibody
    • S6K-alpha-1 antibody
    see all

Images

  • All lanes : Anti-RSK1 p90 (phospho T348) antibody (ab131350) at 1/500 dilution

    Lane 1 : HeLa cell extracts, untreated
    Lane 2 : HeLa cell extracts, treated with UV

    Predicted band size: 82 kDa

  • Immunofluorescentanalysis of methanol fixed HeLa cells labelling RSK1 p90 (phospho T348) with ab131350 antibody at 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RSK1 p90 (phospho T348) with ab131350 at 1/50 dilution (left). The image on the right is treated with blocking peptide.

References

ab131350 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131350.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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