Anti-RSK1 p90 (phospho T348) antibody (ab131350)
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Overview
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Product name
Anti-RSK1 p90 (phospho T348) antibody
See all RSK1 p90 primary antibodies -
Description
Rabbit polyclonal to RSK1 p90 (phospho T348) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic phospho peptide conjugated to KLH from around the phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from Human RSK1 p90 (NP_001006666.1).
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Positive control
- Methanol fixed HeLa cells; HeLa cell extracts (UV treated); Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131350 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa). | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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Database links
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
- Unigene: 149957 Human
see all -
Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Images
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Western blot - Anti-RSK1 p90 (phospho T348) antibody (ab131350)All lanes : Anti-RSK1 p90 (phospho T348) antibody (ab131350) at 1/500 dilution
Lane 1 : HeLa cell extracts, untreated
Lane 2 : HeLa cell extracts, treated with UV
Predicted band size: 82 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T348) antibody (ab131350)Immunofluorescentanalysis of methanol fixed HeLa cells labelling RSK1 p90 (phospho T348) with ab131350 antibody at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho T348) antibody (ab131350)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RSK1 p90 (phospho T348) with ab131350 at 1/50 dilution (left). The image on the right is treated with blocking peptide.
Protocols
Datasheets and documents
References
ab131350 has not yet been referenced specifically in any publications.
