Anti-RSK1 p90 (phospho T348) antibody (ab131350)
Key features and details
- Rabbit polyclonal to RSK1 p90 (phospho T348)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RSK1 p90 (phospho T348) antibody
See all RSK1 p90 primary antibodies -
Description
Rabbit polyclonal to RSK1 p90 (phospho T348) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phospho peptide conjugated to KLH from around the phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from Human RSK1 p90 (NP_001006666.1).
-
Positive control
- Methanol fixed HeLa cells; HeLa cell extracts (UV treated); Human breast carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131350 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa). |
|
IHC-P
1/50 - 1/100. |
|
ICC/IF
1/100 - 1/200. |
Target
-
Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
-
Database links
- Entrez Gene: 6195 Human
- Omim: 601684 Human
- SwissProt: Q15418 Human
- Unigene: 149957 Human
-
Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Images
-
Western blot - Anti-RSK1 p90 (phospho T348) antibody (ab131350)All lanes : Anti-RSK1 p90 (phospho T348) antibody (ab131350) at 1/500 dilution
Lane 1 : HeLa cell extracts, untreated
Lane 2 : HeLa cell extracts, treated with UV
Predicted band size: 82 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T348) antibody (ab131350)Immunofluorescentanalysis of methanol fixed HeLa cells labelling RSK1 p90 (phospho T348) with ab131350 antibody at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho T348) antibody (ab131350)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RSK1 p90 (phospho T348) with ab131350 at 1/50 dilution (left). The image on the right is treated with blocking peptide.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab131350 has not yet been referenced specifically in any publications.