Overview

  • Product name
    Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]
    See all RSK1 p90 primary antibodies
  • Description
    Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363. 80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IP, Dot blotmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RSK1 p90 aa 350-450. The exact sequence is proprietary.

  • Positive control
    • WB: A431 cell lysate. IHC-P: Human cervical carcinoma tissue. WB: NIH/3T3 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/50.
IP 1/40.
Dot blot 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    Images

    • Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
      Left image : Untreated.
      Right image : Treated with Phosphatase.
    • All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/1000 dilution (purified)

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) grown in serum free media for 4 hour swhole cell lysate
      Lane 2 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30minutes whole cell lysate
      Lane 3 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 90 kDa
      Observed band size: 90 kDa



      Blocking/Diluting Buffer and concentration 5% NFDM/TBST

    • Dot blot analysis of RSK1 p90 (phospho T359 + S363) phospho peptide (Lane 1), RSK1 p90 phospho T359 phospho peptide (Lane 2), RSK1 p90 phospho S36 phospho peptide (Lane 3) and RSK1 p90 non-phospho peptide (Lane 4) labeling RSK1 p90 (phospho T359 + S363) phospho peptide with purified ab32413 at a dilution of 1/1000 dilution (2.056ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

      Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .

    • BT549 cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) (ab32413) correlates with an increase in PD 173074 concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilutionand ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution

      Lane 1 : A431 cell lysate untreated.
      Lane 2 : A431 cell lysate treated with EGF.

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    References

    This product has been referenced in:
    • Su L  et al. Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway. Exp Ther Med 15:4185-4190 (2018). Read more (PubMed: 29731817) »
    • Wang X  et al. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway. Cell Death Dis 9:578 (2018). Read more (PubMed: 29784961) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Human Tissue sections (Human fibromatosis)
    Specification
    Human fibromatosis
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 05 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (mouse liver)
    Specification
    mouse liver
    Permeabilization
    Yes - Tween-20
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 25 2014

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Pig Cell lysate - whole cell (PAE cells)
    Specification
    PAE cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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