Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
- Suitable for: WB, IHC-P, IP, Dot blot
- Reacts with: Mouse, Rat, Human
Product nameAnti-RSK1 p90 (phospho T359 + S363) antibody [E238]
See all RSK1 p90 primary antibodies
DescriptionRabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
SpecificityThis antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363. 80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
Tested applicationsSuitable for: WB, IHC-P, IP, Dot blotmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RSK1 p90 aa 350-450. The exact sequence is proprietary.
- WB: A431 and NIH/3T3 cell lysates. IHC-P: Human cervical carcinoma tissue. IP: NIH/3T3 cell lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-380, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Left image : Untreated.
Right image : Treated with Phosphatase.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) grown in serum free media for 4 hour swhole cell lysate
Lane 2 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30minutes whole cell lysate
Lane 3 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Blocking/Diluting Buffer and concentration 5% NFDM/TBST
Purified ab32413 at 1/100 dilution (2µg) immunoprecipitating RSK1 p90 in NIH/3T3 whole cell lysate.
Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) serum starved for 4h, then treated with 20% FBS for 30min whole cell lysate 10µg
Lane 2 (+): ab32413 + NIH/3T3 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab171376 in NIH/3T3 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90 kDa
Lower bands could be cleavage product. (PMID: 29290690)
Dot blot analysis of RSK1 p90 (phospho T359 + S363) phospho peptide (Lane 1), RSK1 p90 phospho T359 phospho peptide (Lane 2), RSK1 p90 phospho S36 phospho peptide (Lane 3) and RSK1 p90 non-phospho peptide (Lane 4) labeling RSK1 p90 (phospho T359 + S363) phospho peptide with purified ab32413 at a dilution of 1/1000 dilution (2.056ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
BT549 cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) (ab32413) correlates with an increase in PD 173074 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilutionand ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution
Lane 1 : A431 cell lysate untreated.
Lane 2 : A431 cell lysate treated with EGF.
Predicted band size: 90 kDa
Observed band size: 90 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32413 has been referenced in 13 publications.
- Mirzoev TM et al. Acute recovery from disuse atrophy: the role of stretch-activated ion channels in the activation of anabolic signaling in skeletal muscle. Am J Physiol Endocrinol Metab 316:E86-E95 (2019). PubMed: 30457911
- Tyganov S et al. An Anabolic Signaling Response of Rat Soleus Muscle to Eccentric Contractions Following Hindlimb Unloading: A Potential Role of Stretch-Activated Ion Channels. Int J Mol Sci 20:N/A (2019). PubMed: 30866432
- Dardaei L et al. SHP2 inhibition restores sensitivity in ALK-rearranged non-small-cell lung cancer resistant to ALK inhibitors. Nat Med 24:512-517 (2018). PubMed: 29505033
- Su L et al. Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway. Exp Ther Med 15:4185-4190 (2018). PubMed: 29731817
- Wang X et al. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway. Cell Death Dis 9:578 (2018). PubMed: 29784961