Key features and details
- Rabbit polyclonal to RSK1 p90 (phospho T359)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RSK1 p90 (phospho T359) antibody
See all RSK1 p90 primary antibodies
DescriptionRabbit polyclonal to RSK1 p90 (phospho T359)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
Synthetic peptide within Human RSK1 p90 (internal sequence) (phospho T359). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q15418
- WB: A431, starved for 24 hours and treated with 200 ng/ml EGF for 15 minutes, whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 82 kDa.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-380, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
All lanes : Anti-RSK1 p90 (phospho T359) antibody (ab226996) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line), starved for 24 hours, whole cell extract
Lane 2 : A431, starved for 24 hours and treated with 200 ng/ml EGF for 15 minutes, whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 82 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226996 has not yet been referenced specifically in any publications.