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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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Anti-RSK1 p90 (phospho T573) antibody (ab227161)

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Western blot - Anti-RSK1 p90 (phospho T573) antibody (ab227161)

    Key features and details

    • Rabbit polyclonal to RSK1 p90 (phospho T573)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human RSK1 p90 protein (ab159427)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-RSK1 p90 (phospho T573) antibody
      See all RSK1 p90 primary antibodies
    • Description

      Rabbit polyclonal to RSK1 p90 (phospho T573)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human RSK1 p90 (internal sequence) (phospho T573). The exact sequence is proprietary. Conjugated to a carrier protein.
      Database link: Q15418

    • Positive control

      • WB: A431 whole cell extract.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • A431 whole cell lysate (ab30132)
      • A431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human RSK1 p90 protein (ab159427)

    Applications

    Our Abpromise guarantee covers the use of ab227161 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

    Target

    • Function

      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Autophosphorylated on Ser-380, as part of the activation process.
    • Target information above from: UniProt accession Q15418 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6195 Human
      • Omim: 601684 Human
      • SwissProt: Q15418 Human
      • Unigene: 149957 Human
      • Alternative names

        • 90 kDa ribosomal protein S6 kinase 1 antibody
        • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
        • dJ590P13.1 antibody
        • EC 2.7.11.1 antibody
        • HU 1 antibody
        • HU1 antibody
        • KS6A1_HUMAN antibody
        • MAP kinase activated protein kinase 1a antibody
        • MAP kinase-activated protein kinase 1a antibody
        • MAPK-activated protein kinase 1a antibody
        • MAPKAP kinase 1a antibody
        • MAPKAPK-1a antibody
        • MAPKAPK1A antibody
        • MGC79981 antibody
        • Mitogen-activated protein kinase-activated protein kinase 1A antibody
        • OTTHUMP00000004113 antibody
        • p90 RSK1 antibody
        • p90-RSK 1 antibody
        • p90rsk antibody
        • p90RSK1 antibody
        • p90S6K antibody
        • pp90RSK1 antibody
        • Ribosomal protein S6 kinase 90kD 1 antibody
        • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
        • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
        • Ribosomal protein S6 kinase alpha 1 antibody
        • Ribosomal protein S6 kinase alpha-1 antibody
        • Ribosomal protein S6 kinase polypeptide 1 antibody
        • Ribosomal S6 kinase 1 antibody
        • RPS6K1 alpha antibody
        • rps6ka antibody
        • Rps6ka1 antibody
        • RSK 1 antibody
        • RSK 1 p90 antibody
        • RSK antibody
        • RSK-1 antibody
        • RSK1 antibody
        • RSK1p90 antibody
        • S6K alpha 1 antibody
        • S6K-alpha-1 antibody
        see all

      Images

      • Western blot - Anti-RSK1 p90 (phospho T573) antibody (ab227161)
        Western blot - Anti-RSK1 p90 (phospho T573) antibody (ab227161)
        All lanes : Anti-RSK1 p90 (phospho T573) antibody (ab227161) at 1/1000 dilution

        Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
        Lane 2 : A431 whole cell extract treated with CIP

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit IgG

        Developed using the ECL technique.

        Predicted band size: 83 kDa



        10% SDS-PAGE

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab227161? Please let us know so that we can cite the reference in this datasheet.

      ab227161 has not yet been referenced specifically in any publications.

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