Recombinant Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-RSK1 p90 (phospho T573) antibody [EP2133Y]
See all RSK1 p90 primary antibodies -
Description
Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RSK1 p90 (phospho T573). The exact sequence is proprietary.
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Positive control
- K562 cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2133Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab62324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsAutophosphorylated on Ser-380, as part of the activation process. - Information by UniProt
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Database links
- Entrez Gene: 6195 Human
- Omim: 601684 Human
- SwissProt: Q15418 Human
- Unigene: 149957 Human
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Alternative names
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
see all
Images
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![Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.com/ps/products/62/ab62324/Images/ab62324-257528-anti-rsk1-p90-phospho-t573-antibody-ep2133y-western-blot.jpg)
Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.com/ps/products/62/ab62324/Images/ab62324-257435-anti-rsk1-p90-phospho-t573-antibody-ep2133y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)](https://www.abcam.com/ps/products/62/ab62324/Images/ab62324-75622-anti-rsk1-p90-phospho-t573-antibody-ep2133y-western-blot.jpg)
Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)
Lane 1 : K562 cell lysate; untreated
Lane 2 : K562 cell lysate; treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Beta Tubulin has been included as a loading control.
Datasheets and documents
References
This product has been referenced in:
- Chaikuad A et al. A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nat Chem Biol 10:853-60 (2014). Read more (PubMed: 25195011) »
- Talbot AL et al. Aconitase regulation of erythropoiesis correlates with a novel licensing function in erythropoietin-induced ERK signaling. PLoS One 6:e23850 (2011). WB . Read more (PubMed: 21887333) »
