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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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RecombinantRabMAb

Recombinant Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)

  • Datasheet
Submit a review Submit a question References (3)

Product price, shipping and contact information

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Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
  • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
  • Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
  • Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573)
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human RSK1 p90 protein (ab159427)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-ELK1 antibody [E277] - BSA and Azide free (ab227114)

View more associated products

Overview

  • Product name

    Anti-RSK1 p90 (phospho T573) antibody [EP2133Y]
    See all RSK1 p90 primary antibodies
  • Description

    Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP2133Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] - BSA and Azide free (ab209965)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human RSK1 p90 protein (ab159427)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
ICC/IF
1/100 - 1/500.
WB
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Notes
ICC/IF
1/100 - 1/500.
WB
1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Autophosphorylated on Ser-380, as part of the activation process.
    • Target information above from: UniProt accession Q15418 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6195 Human
      • Omim: 601684 Human
      • SwissProt: Q15418 Human
      • Unigene: 149957 Human
      • Alternative names

        • 90 kDa ribosomal protein S6 kinase 1 antibody
        • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
        • dJ590P13.1 antibody
        • EC 2.7.11.1 antibody
        • HU 1 antibody
        • HU1 antibody
        • KS6A1_HUMAN antibody
        • MAP kinase activated protein kinase 1a antibody
        • MAP kinase-activated protein kinase 1a antibody
        • MAPK-activated protein kinase 1a antibody
        • MAPKAP kinase 1a antibody
        • MAPKAPK-1a antibody
        • MAPKAPK1A antibody
        • MGC79981 antibody
        • Mitogen-activated protein kinase-activated protein kinase 1A antibody
        • OTTHUMP00000004113 antibody
        • p90 RSK1 antibody
        • p90-RSK 1 antibody
        • p90rsk antibody
        • p90RSK1 antibody
        • p90S6K antibody
        • pp90RSK1 antibody
        • Ribosomal protein S6 kinase 90kD 1 antibody
        • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
        • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
        • Ribosomal protein S6 kinase alpha 1 antibody
        • Ribosomal protein S6 kinase alpha-1 antibody
        • Ribosomal protein S6 kinase polypeptide 1 antibody
        • Ribosomal S6 kinase 1 antibody
        • RPS6K1 alpha antibody
        • rps6ka antibody
        • Rps6ka1 antibody
        • RSK 1 antibody
        • RSK 1 p90 antibody
        • RSK antibody
        • RSK-1 antibody
        • RSK1 antibody
        • RSK1p90 antibody
        • S6K alpha 1 antibody
        • S6K-alpha-1 antibody
        see all

      Images

      • Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)

        Lane 1 : Untreated Ramos whole cell lysate
        Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 83 kDa
        Observed band size: 83 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)

        Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      • Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        Western blot - Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)

        Lane 1 : K562 cell lysate; untreated
        Lane 2 : K562 cell lysate; treated with TPA

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution

        Predicted band size: 83 kDa
        Observed band size: 83 kDa



        Beta Tubulin has been included as a loading control.
      • Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
        Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (3)

      Publishing research using ab62324? Please let us know so that we can cite the reference in this datasheet.

      ab62324 has been referenced in 3 publications.

      • Chaikuad A  et al. A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nat Chem Biol 10:853-60 (2014). PubMed: 25195011
      • Talbot AL  et al. Aconitase regulation of erythropoiesis correlates with a novel licensing function in erythropoietin-induced ERK signaling. PLoS One 6:e23850 (2011). WB . PubMed: 21887333
      • Burd NA  et al. Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol 588:3119-30 (2010). PubMed: 20581041

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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