Overview

  • Product name

  • Description

    Rabbit polyclonal to RSK3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human RSK3 aa 1-50 (N terminal). The exact sequence is proprietary. NP_066958.2.
    Sequence:

    MDLSMKKFAV RRFFSVYLRR KSRSKSSSLS RLEEEGVVKE IDISHHVKEG


    Database link: Q15349

  • Positive control

    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab185999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
    • Tissue specificity

      Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
      N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 2 antibody
      • HU 2 antibody
      • KS6A2_HUMAN antibody
      • MAP kinase activated protein kinase 1c antibody
      • MAP kinase-activated protein kinase 1c antibody
      • MAPK-activated protein kinase 1c antibody
      • MAPKAP kinase 1c antibody
      • MAPKAPK-1c antibody
      • MAPKAPK1C antibody
      • Mitogen.activated protein kinase-activated protein kinase 1C antibody
      • p90 RSK3 antibody
      • p90-RSK 2 antibody
      • p90RSK2 antibody
      • pp90RSK3 antibody
      • Ribosomal protein S6 kinase alpha-2 antibody
      • ribosomal protein S6 kinase, 90kDa, polypeptide 2 antibody
      • Ribosomal S6 kinase 3 antibody
      • RPS6KA2 antibody
      • RSK 3 antibody
      • RSK antibody
      • RSK-3 antibody
      • S6K alpha antibody
      • S6K alpha 2 antibody
      • S6K-alpha-2 antibody
      see all

    Images

    • Detection of Human RSK by Western Blot of Immunoprecipitates of HeLa
      whole cell lysate (1 mg for IP; 20% of IP loaded) .
      Lane 1: ab185999 used for IP at 6 μg/mg lysate.
      Lane 2: Control IgG.

      For blotting an anti-RSK antibody was used at 1 μg/ml.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab185999 has not yet been referenced specifically in any publications.

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