Key features and details
- Rabbit polyclonal to RSK3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RSK3 antibody
See all RSK3 primary antibodies
DescriptionRabbit polyclonal to RSK3
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Common marmoset, Orangutan
- HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab185999 was affinity purified using an epitope specific to RSK3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab185999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSerine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Tissue specificityWidely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsActivated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 2 antibody
- HU 2 antibody
- KS6A2_HUMAN antibody
Detection of Human RSK by Western Blot of Immunoprecipitates of HeLa
whole cell lysate (1 mg for IP; 20% of IP loaded) .
Lane 1: ab185999 used for IP at 6 μg/mg lysate.
Lane 2: Control IgG.
For blotting an anti-RSK antibody was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab185999 has not yet been referenced specifically in any publications.