Key features and details
- Rabbit polyclonal to RSK3 (phospho T356 + S360)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RSK3 (phospho T356 + S360) antibody
See all RSK3 primary antibodies
DescriptionRabbit polyclonal to RSK3 (phospho T356 + S360)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human RSK3 (internal sequence) (phospho T356 + S360). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q15349
- WB: Serum starved A431 cells treated with EGF whole cell extracts. A431 whole cell extracts.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 83 kDa.|
FunctionSerine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Tissue specificityWidely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsActivated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 2 antibody
- HU 2 antibody
- KS6A2_HUMAN antibody
All lanes : Anti-RSK3 (phospho T356 + S360) antibody (ab226952) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line), starved for 24 hours, whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line), starved for 24 hours and treated with 200 ng/ml EGF for 15 minutes, whole cell extract
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 83 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226952 has not yet been referenced specifically in any publications.