Product nameAnti-RSK4 (phospho S232) antibody [EP1524Y]
See all RSK4 primary antibodies
DescriptionRabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).
This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK4 aa 200-300 (phospho S232).
Database link: Q9UK32
- Hela cell lysate and human muscle tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab81290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-389, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 6 antibody
- KS6A6_HUMAN antibody
- p90 RSK 6 antibody
All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution
Lane 1 : Hela cell lysate untreated
Lane 2 : Hela cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Direct ELISA antigen dose-response curve using ab81290.
Primary antibody: ab81290 (0-1000 ng/ml).
RSK3 (phospho S218) phopspho peptide
RSK3 non-phopspho peptide
RSK4 (phospho S232) phopspho peptide
RSK4 non-phopspho peptide
BSA-branched PEG 40k (0-100 ng/ml).
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
ab81290 has not yet been referenced specifically in any publications.