Overview

  • Product name

    Anti-RSK4 (phospho S232) antibody [EP1524Y]
    See all RSK4 primary antibodies
  • Description

    Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
  • Host species

    Rabbit
  • Specificity

    Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).

    This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK4 aa 200-300 (phospho S232).
    Database link: Q9UK32

  • Positive control

    • Hela cell lysate and human muscle tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1524Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications

      Autophosphorylated on Ser-389, as part of the activation process.
    • Information by UniProt
    • Database links

    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 6 antibody
      • KS6A6_HUMAN antibody
      • p90 RSK 6 antibody
      • p90-RSK 6 antibody
      • p90RSK6 antibody
      • pp90RSK4 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 6 antibody
      • Ribosomal protein S6 kinase alpha 6 antibody
      • Ribosomal protein S6 kinase alpha-6 antibody
      • Ribosomal S6 kinase 4 antibody
      • rps6ka6 antibody
      • RSK 4 antibody
      • RSK-4 antibody
      • S6K-alpha-6 antibody
      see all

    Images

    • All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution

      Lane 1 : Hela cell lysate untreated
      Lane 2 : Hela cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled anti-rabbit at 1/2000 dilution

      Predicted band size: 84 kDa
      Observed band size: 84 kDa

    • ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Direct ELISA antigen dose-response curve using ab81290.

      Primary antibody: ab81290 (0-1000 ng/ml).

      Antigens:

      RSK3 (phospho S218) phopspho peptide

      RSK3 non-phopspho peptide

      RSK4 (phospho S232) phopspho peptide

      RSK4 non-phopspho peptide

      BSA-branched PEG 40k (0-100 ng/ml).

      Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

    • Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    References

    ab81290 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab81290.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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