Key features and details
- Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
- Suitable for: WB, IHC-P, ELISA, Dot blot
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RSK4 (phospho S232) antibody [EP1524Y]
See all RSK4 primary antibodies
DescriptionRabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).
This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.
Tested applicationsSuitable for: WB, IHC-P, ELISA, Dot blotmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK4 aa 200-300 (phospho S232).
Database link: Q9UK32
- Hela cell lysate and human muscle tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab81290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
modificationsAutophosphorylated on Ser-389, as part of the activation process.
- Information by UniProt
- 90 kDa ribosomal protein S6 kinase 6 antibody
- KS6A6_HUMAN antibody
- p90 RSK 6 antibody
All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution
Lane 1 : Hela cell lysate untreated
Lane 2 : Hela cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Direct ELISA antigen dose-response curve using ab81290.
Primary antibody: ab81290 (0-1000 ng/ml).
RSK3 (phospho S218) phopspho peptide
RSK3 non-phopspho peptide
RSK4 (phospho S232) phopspho peptide
RSK4 non-phopspho peptide
BSA-branched PEG 40k (0-100 ng/ml).
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
ab81290 has not yet been referenced specifically in any publications.