Anti-L12 antibody (ab202978)
Key features and details
- Rabbit polyclonal to L12
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-L12 antibody
See all L12 primary antibodies -
Description
Rabbit polyclonal to L12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human L12 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O76021 -
Positive control
- Rat brain lysate; Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 55 kDa.
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|
IHC-P |
1/100 - 1/500.
(or 1/50 - 1/200 if using a fluorescent secondary antibody). |
Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/500. (or 1/50 - 1/200 if using a fluorescent secondary antibody). |
Target
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Tissue specificity
Placenta. -
Sequence similarities
Belongs to the ribosomal protein L1P family. Highly divergent. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 26156 Human
- Entrez Gene: 302898 Rat
- Omim: 615874 Human
- SwissProt: O76021 Human
- Unigene: 401842 Human
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Alternative names
- CATX 11 antibody
- CATX-11 antibody
- CATX11 antibody
see all
Images
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All lanes : Anti-L12 antibody (ab202978) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?12% SDS-PAGE
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling L12 with ab202978 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202978 has not yet been referenced specifically in any publications.