Product nameAnti-RSPH3 antibody
DescriptionRabbit polyclonal to RSPH3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human testis tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesBelongs to the flagellar radial spoke RSP3 family.
- Information by UniProt
- dJ111C20.1 antibody
- Radial spoke 3 homolog (Chlamydomonas) antibody
- Radial spoke head 3 homolog antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122535 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122535, at 1/250 dilution, staining RSPH3 in paraffin-embedded Human testis tissue by Immunohistochemistry, showing moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
All lanes : Anti-RSPH3 antibody (ab122535) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human Liver lysate
Lane 6 : Human Tonsil lysate
ab122535 has not yet been referenced specifically in any publications.