Key features and details
- Rabbit polyclonal to RSPH9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RSPH9 antibody
See all RSPH9 primary antibodies
DescriptionRabbit polyclonal to RSPH9
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human RSPH9 aa 62-171.
Database link: Q9H1X1
- WB: Mouse liver and skeletal muscle lysates. IHC-P: Human melanoma cancer and testis tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionProbable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk, which is attached to a subfiber of the outer doublet microtubule, and a bulbous head, which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.
Involvement in diseaseDefects in RSPH9 are the cause of primary ciliary dyskinesia type 12 (CILD12) [MIM:612650]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Sequence similaritiesBelongs to the flagellar radial spoke RSP9 family.
Cellular localizationCytoplasm > cytoskeleton > cilium axoneme. Radial spoke.
- Information by UniProt
- C6orf206 antibody
- Chromosome 6 open reading frame 206 antibody
- CILD12 antibody
All lanes : Anti-RSPH9 antibody (ab223045) at 1/2000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse skeletal muscle lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Paraffin-embedded human testis tissue stained for RSPH9 using ab223045 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human melanoma cancer tissue stained for RSPH9 using ab223045 at 1/100 dilution in immunohistochemical analysis.
ab223045 has not yet been referenced specifically in any publications.