Overview

  • Product name

    Anti-RSPO3 antibody - C-terminal
    See all RSPO3 primary antibodies
  • Description

    Rabbit polyclonal to RSPO3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within Human RSPO3 aa 202-251 (C terminal). The exact sequence is proprietary.
    Sequence:

    QRKKCQKGERGKKGRERKRKKPNKGESKEAIPDSKSLESSKEIPEQRENK


    Database link: NP_116173

  • Positive control

    • HT1080 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab171010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.

Target

  • Function

    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
  • Tissue specificity

    Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.
  • Sequence similarities

    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • Domain

    The FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRISTIN1 antibody
    • hPWTSR antibody
    • hRspo3 antibody
    • Protein with TSP type-1 repeat antibody
    • PWTSR antibody
    • R-spondin 3 homolog antibody
    • R-spondin-3 [Precursor] antibody
    • R-spondin-3 antibody
    • Roof plate specific spondin-3 antibody
    • Roof plate-specific spondin-3 antibody
    • RSPO3 antibody
    • RSPO3_HUMAN antibody
    • Thrombospondin type I domain containing 2 antibody
    • Thrombospondin type-1 domain-containing protein 2 antibody
    • THSD2 antibody
    see all

Images

  • Anti-RSPO3 antibody - C-terminal (ab171010) at 1 µg/ml + HT1080 whole cell lysate at 10 µg

    Predicted band size: 31 kDa

References

ab171010 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Small intestine)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
Small intestine
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 23 2018

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