Key features and details
- Rabbit polyclonal to RSU1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RSU1 antibody
See all RSU1 primary antibodies
DescriptionRabbit polyclonal to RSU1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 32 kDa.|
FunctionPotentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro.
Sequence similaritiesContains 7 LRR (leucine-rich) repeats.
- Information by UniProt
- FLJ31034 antibody
- Ras suppressor protein 1 antibody
- Ras suppressor protein 1 isoform 1 antibody
Detection of RSU1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217061 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217061 was used at 0.4µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-RSU1 antibody (ab217061) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH 3T3 whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 10 seconds
ab217061 has not yet been referenced specifically in any publications.