Key features and details
- Rabbit polyclonal to RTF1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RTF1 antibody
See all RTF1 primary antibodies
DescriptionRabbit polyclonal to RTF1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).
- HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
Sequence similaritiesContains 1 Plus3 domain.
DomainThe Plus3 domain mediates single-stranded DNA-binding.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
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All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: RTF1 antibody (ab99362) at 1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds
Predicted band size : 77 kDa
ab99362 has been referenced in 1 publication.
- Eirin A et al. Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells. Sci Rep 6:36120 (2016). PubMed: 27786293