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Rabbit polyclonal to RTF1
Predicted to work with:
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
Contains 1 Plus3 domain.
The Plus3 domain mediates single-stranded DNA-binding.
Nucleus > nucleoplasm.
Information by UniProt
GTL 7 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (ab99362)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-RTF1 antibody (ab99362)
All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 77 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-RTF1 antibody (ab99362)
1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: RTF1 antibody (ab99362) at 1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds Predicted band size : 77 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"