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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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RecombinantRabMAb

Recombinant Anti-RTF1 antibody [EPR13615] (ab184570)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Anti-RTF1 antibody [EPR13615] (ab184570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13615] to RTF1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-RTF1 antibody [EPR13615]
    See all RTF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13615] to RTF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, A431, HepG2 or HeLa lysate, Human papillary adenocarcinoma of thyroid or stomach tissue, HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13615
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Elongation factors
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Yeast
    • Saccharomyces spp
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • Anti-RTF1 antibody [EPR13615] - BSA and Azide free (ab250771)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.
  • Sequence similarities

    Contains 1 Plus3 domain.
  • Domain

    The Plus3 domain mediates single-stranded DNA-binding.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Target information above from: UniProt accession Q92541 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23168 Human
    • Entrez Gene: 76246 Mouse
    • Entrez Gene: 366169 Rat
    • Omim: 611633 Human
    • SwissProt: Q92541 Human
    • SwissProt: A2AQ19 Mouse
    • SwissProt: Q08EC5 Mouse
    • Unigene: 511096 Human
    • Unigene: 33437 Mouse
    see all
  • Alternative names

    • CDG1N antibody
    • GTL 7 antibody
    • GTL7 antibody
    • KIAA0252 antibody
    • Ortholog of mouse gene trap locus 7 antibody
    • RNA polymerase associated protein RTF1 homolog antibody
    • RNA polymerase-associated protein RTF1 homolog antibody
    • RTF 1 antibody
    • RTF1 antibody
    • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
    • RTF1_HUMAN antibody
    • rtf1L antibody
    • YGL244W antibody
    see all

Images

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570) at 1/5000 dilution + HeLa lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1  with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution

    Lane 1 : 293 lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab184570? Please let us know so that we can cite the reference in this datasheet.

ab184570 has not yet been referenced specifically in any publications.

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