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RecombinantRabMAb

Recombinant Anti-RTF1 antibody [EPR13615] (ab184570)

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Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
  • Anti-RTF1 antibody [EPR13615] (ab184570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13615] to RTF1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RTF1 antibody [EPR13615]
    See all RTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13615] to RTF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, A431, HepG2 or HeLa lysate, Human papillary adenocarcinoma of thyroid or stomach tissue, HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.

  • Sequence similarities

    Contains 1 Plus3 domain.

  • Domain

    The Plus3 domain mediates single-stranded DNA-binding.

  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CDG1N antibody
    • GTL 7 antibody
    • GTL7 antibody
    • KIAA0252 antibody
    • Ortholog of mouse gene trap locus 7 antibody
    • RNA polymerase associated protein RTF1 homolog antibody
    • RNA polymerase-associated protein RTF1 homolog antibody
    • RTF 1 antibody
    • RTF1 antibody
    • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
    • RTF1_HUMAN antibody
    • rtf1L antibody
    • YGL244W antibody
    see all

Images

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570) at 1/5000 dilution + HeLa lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1  with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution

    Lane 1 : 293 lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570)

References (0)

Publishing research using ab184570? Please let us know so that we can cite the reference in this datasheet.

ab184570 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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