Recombinant
RabMAb

Recombinant Anti-RTF1 antibody [EPR13615] (ab184570)

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Overview

  • Product name

    Anti-RTF1 antibody [EPR13615]
    See all RTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13615] to RTF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RTF1 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92541

  • Positive control

    • 293, A431, HepG2 or HeLa lysate, Human papillary adenocarcinoma of thyroid or stomach tissue, HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab184570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA.

  • Sequence similarities

    Contains 1 Plus3 domain.

  • Domain

    The Plus3 domain mediates single-stranded DNA-binding.

  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CDG1N antibody
    • GTL 7 antibody
    • GTL7 antibody
    • KIAA0252 antibody
    • Ortholog of mouse gene trap locus 7 antibody
    • RNA polymerase associated protein RTF1 homolog antibody
    • RNA polymerase-associated protein RTF1 homolog antibody
    • RTF 1 antibody
    • RTF1 antibody
    • Rtf1 Paf1/RNA polymerase II complex component homolog antibody
    • RTF1_HUMAN antibody
    • rtf1L antibody
    • YGL244W antibody
    see all

Images

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570) at 1/5000 dilution + HeLa lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1  with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution

    Lane 1 : 293 lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

References

ab184570 has not yet been referenced specifically in any publications.

Publishing research using ab184570? Please let us know so that we can cite the reference in this datasheet.

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