Recombinant Anti-RTF1 antibody [EPR13615] (ab184570)
![Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-217132-anti-rtf1-antibody-epr13615-western-blot.jpg "Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13615] to RTF1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-RTF1 antibody [EPR13615]
See all RTF1 primary antibodies -
Description
Rabbit monoclonal [EPR13615] to RTF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- 293, A431, HepG2 or HeLa lysate, Human papillary adenocarcinoma of thyroid or stomach tissue, HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13615 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/100. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA. -
Sequence similarities
Contains 1 Plus3 domain. -
Domain
The Plus3 domain mediates single-stranded DNA-binding. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 23168 Human
- Entrez Gene: 76246 Mouse
- Entrez Gene: 366169 Rat
- Omim: 611633 Human
- SwissProt: Q92541 Human
- SwissProt: A2AQ19 Mouse
- SwissProt: Q08EC5 Mouse
- Unigene: 511096 Human
see all -
Alternative names
- CDG1N antibody
- GTL 7 antibody
- GTL7 antibody
see all
Images
-
Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)Anti-RTF1 antibody [EPR13615] (ab184570) at 1/5000 dilution + HeLa lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-217135-anti-rtf1-antibody-epr13615-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-217134-anti-rtf1-antibody-epr13615-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-217133-anti-rtf1-antibody-epr13615-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-217131-anti-rtf1-antibody-epr13615-western-blot.jpg)
Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution
Lane 1 : 293 lysate
Lane 2 : A431 lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa -
![Anti-RTF1 antibody [EPR13615] (ab184570)](https://www.abcam.com/ps/products/184/ab184570/Images/ab184570-6-benefits-of-recombinant-antibodies.png)
Anti-RTF1 antibody [EPR13615] (ab184570)
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab184570 has not yet been referenced specifically in any publications.
