Recombinant Anti-RTF1 antibody [EPR13615] - BSA and Azide free (ab250771)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13615] to RTF1 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RTF1 antibody [EPR13615] - BSA and Azide free
See all RTF1 primary antibodies -
Description
Rabbit monoclonal [EPR13615] to RTF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250771 is the carrier-free version of ab184570.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13615 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. |
Target
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Function
Plays a role in transcription-coupled histone modification. Plays a role in regulation of transcription. Required for maximal induction of heat-shock genes (By similarity). Binds single-stranded DNA. -
Sequence similarities
Contains 1 Plus3 domain. -
Domain
The Plus3 domain mediates single-stranded DNA-binding. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23168 Human
- Entrez Gene: 76246 Mouse
- Entrez Gene: 366169 Rat
- Omim: 611633 Human
- SwissProt: Q92541 Human
- SwissProt: A2AQ19 Mouse
- SwissProt: Q08EC5 Mouse
- Unigene: 511096 Human
see all -
Alternative names
- CDG1N antibody
- GTL 7 antibody
- GTL7 antibody
see all
Images
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This data was developed using ab184570, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).
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This data was developed using ab184570, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab184570, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution
Lane 1 : 293 lysate
Lane 2 : A431 lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDaThis data was developed using ab184570, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250771 has not yet been referenced specifically in any publications.