Overview

  • Product name

    RTIL™ 13, dynamin I and II inhibitor
  • Description

    Potent dynamin I and II inhibitor
  • Biological description

    Potent dynamin I and II inhibitor. Inhibits dynamin I GTPase (IC50 = 2.3 μM) and targets pleckstrin homology (PH) (lipid binding) domain. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 9.3 and 7.1 μM, respectively).
  • Purity

    > 98%
  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. RTIL™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • CAS Number

    1009376-10-6
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab13847 staining active caspase 3 in HeLa cells treated with RTIL-13™ (ab120465), by ICC/IF. Increase in active caspase 3 expression correlates with increased concentration of RTIL-13™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120465 (RTIL-13™) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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