Overview

  • Product name

    Anti-RTKN/Rhotekin antibody
    See all RTKN/Rhotekin primary antibodies
  • Description

    Rabbit polyclonal to RTKN/Rhotekin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human RTKN/Rhotekin aa 1-141.
    Database link: Q9BST9

  • Positive control

    • WB: 293T, A431, Jurkat, Raji and mouse brain whole cell lysates. A431 cells. ICC/IF: Rhotekin-transfected HeLa cells.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as RTKN

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/5000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa

  • Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Stained with ab154954 diluted at 1/500 (green). Nuclear staining using Hoechst 33342 (blue).

  • All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : human Rhotekin-transfected 293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa

  • Anti-RTKN/Rhotekin antibody (ab154954) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • Anti-RTKN/Rhotekin antibody (ab154954) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling RTKN/Rhotekin with ab154954 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

References

This product has been referenced in:

See 1 Publication for this product

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