Key features and details
- Rabbit polyclonal to RTKN/Rhotekin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RTKN/Rhotekin antibody
See all RTKN/Rhotekin primary antibodies
DescriptionRabbit polyclonal to RTKN/Rhotekin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human RTKN/Rhotekin aa 304-563.
PLCMTQPTASGTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEE PLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEVTHTLQTESREA LQSWMEALWQLFFDMSQWKQCCDEIMKIETPAPRKPPQALAKQGSLYHEM AIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAP APDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKG QPRTWLQSPV
Database link: Q9BST9
- IHC-P: Human prostate cancer and testis tissues. WB: A431, HepG2, MCF-7 and mouse brain lysates.
This product was previously labelled as RTKN
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab234866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||Use a concentration of 4.66 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionMediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures.
Tissue specificityHighly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines.
Sequence similaritiesContains 1 PH domain.
Contains 1 REM (Hr1) repeat.
- Information by UniProt
- Rhotekin antibody
- Rtkn antibody
- RTKN_HUMAN antibody
- RTKN1 antibody
All lanes : Anti-RTKN/Rhotekin antibody (ab234866) at 4.66 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : Mouse brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63 kDa
Paraffin-embedded human testis tissue stained for RTKN/Rhotekin using ab234866 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate cancer tissue stained for RTKN/Rhotekin using ab234866 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234866 has not yet been referenced specifically in any publications.